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NativeFolder Bacterial Culture Medium

From: $595.00$350.00

Catalog #: K1010004 (1L)

NativeFolderTM is a bacterial culture medium specially formulated to promote the folding and the expression of soluble recombinant  proteins in E. coli. NativeFolderTM contains proprietary compounds that prevent protein aggregation and misfolding during protein expression. Don’t waste your time experimenting with the renaturation of inclusion bodies. Use NativeFolderTM and express your correctly folded target protein in the soluble fraction. Simply add water to the provided mix, autoclave and filter sterilize. Recombinant proteins of up to 110 kDa were successfully expressed as soluble fractions using NativeFolderTM.

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Description

NativeFolder Bacterial Culture Medium

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Catalog # K1010004
Content NativeFolderTM Mix A

NativeFolderTM Mix B

Instructions

Keywords Culture Medium, folding, solubilization, recombinant proteins, soluble protein expression, expression insoluble proteins, purification of soluble protein from e.coli
Storage Room temperature
Expression Protocol 1- Dissolve NativeFolderTM Mix A in 0.75 L of di-ionized water, stir until completely dissolved and autoclave.

2- Dissolve NativeFolderTM Mix B in 0.25 L of di-ionized water, stir until completely dissolved filter sterilize before use (DO NOT AUTOCLAVE).

3- Combine 0.75 L of NativeFolderTM Mix A with 0.25 L of NativeFolder Mix B to make 1.0 L of NativeFolderTM Culture Medium. Follow Steps 4-10.

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References

  • Packiam KAR, Ramanan RN, Ooi CW, Krishnaswamy L, Tey BT. Stepwise optimization of recombinant protein production in Escherichia coli utilizing computational and experimental approaches. Appl Microbiol Biotechnol. 2020 Apr;104(8):3253-3266.
  • Satheeshkumar PK. Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update. Mol Biotechnol. 2020 Mar;62(3):151-167.
  • Smolskaya S, Logashina YA, Andreev YA. Escherichia coli Extract-Based Cell-Free Expression System as an Alternative for Difficult-to-Obtain Protein Biosynthesis. Int J Mol Sci. 2020 Jan 31;21(3). pii: E928.
  • Lipinski DM. A Comparison of Inducible Gene Expression Platforms: Implications for Recombinant Adeno-Associated Virus (rAAV) Vector-Mediated Ocular Gene Therapy. Adv Exp Med Biol. 2019;1185:79-83.
  • Powell N, Pantazi E, Pavlidis P, Tsakmaki A, Li K, Yang F, Parker A, Pin C, Cozzetto D, Minns D, Stolarczyk E, Saveljeva S, Mohamed R, Lavender P, Afzali B, Digby-Bell J, Tjir-Li T, Kaser A, Friedman J, MacDonald TT, Bewick GA, Lord GM. Interleukin-22 orchestrates a pathological endoplasmic reticulum stress response transcriptional programme in colonic epithelial cells. Gut. 2020 Mar;69(3):578-590.
  • Temporini C, Colombo R, Calleri E, Tengattini S, Rinaldi F, Massolini G. Chromatographic tools for plant-derived recombinant antibodies purification and characterization. J Pharm Biomed Anal. 2020 Feb 5;179:112920.

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