NativeFolder Bacterial Culture Medium

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Nativefolder Expression of Soluble Proteins
NativeFolder Bacterial Culture Medium
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NativeFolder Bacterial Culture Medium

Original price was: $795.00.Current price is: $295.00.

Catalog #: K1010004 (1L)

NativeFolder Bacterial Culture Medium (1L) is a unique bacterial culture medium formulated to promote the folding and the expression of soluble recombinant proteins in E. coli. NativeFolder contains proprietary compounds that prevent protein aggregation and misfolding during protein expression. This product is supplied as two powdered mixes to be reconsituted with di-water and combined to produce 1L of bacterial culture medium. Large bulk volumes are available at large discounts.

Live Enquiry about this product via Text/SMS: 1-858-900-3210 (8 am – 8 pm PST)

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SKU: K1010004 Categories: , Tag:

Product Description

4.8/5 - (127 votes)

NativeFolder Bacterial Culture Medium

Catalog # K1010004
Content NativeFolderTM Mix A

NativeFolderTM Mix B

Instructions
Keywords Culture Medium, folding, solubilization, recombinant proteins, soluble protein expression, expression insoluble proteins, purification of soluble protein from e.coli
Storage Room temperature
Expression Protocol 1- Dissolve NativeFolderTM Mix A in 0.75 L of di-ionized water, stir until completely dissolved and autoclave.

2- Dissolve NativeFolderTM Mix B in 0.25 L of di-ionized water, stir until completely dissolved filter sterilize before use (DO NOT AUTOCLAVE).

3- Combine 0.75 L of NativeFolderTM Mix A with 0.25 L of NativeFolder Mix B to make 1.0 L of NativeFolderTM Culture Medium. Follow Steps 4-10.
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References

  • Packiam KAR, Ramanan RN, Ooi CW, Krishnaswamy L, Tey BT. Stepwise optimization of recombinant protein production in Escherichia coli utilizing computational and experimental approaches. Appl Microbiol Biotechnol. 2020 Apr;104(8):3253-3266.
  • Satheeshkumar PK. Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update. Mol Biotechnol. 2020 Mar;62(3):151-167.
  • Smolskaya S, Logashina YA, Andreev YA. Escherichia coli Extract-Based Cell-Free Expression System as an Alternative for Difficult-to-Obtain Protein Biosynthesis. Int J Mol Sci. 2020 Jan 31;21(3). pii: E928.
  • Lipinski DM. A Comparison of Inducible Gene Expression Platforms: Implications for Recombinant Adeno-Associated Virus (rAAV) Vector-Mediated Ocular Gene Therapy. Adv Exp Med Biol. 2019;1185:79-83.
  • Powell N, Pantazi E, Pavlidis P, Tsakmaki A, Li K, Yang F, Parker A, Pin C, Cozzetto D, Minns D, Stolarczyk E, Saveljeva S, Mohamed R, Lavender P, Afzali B, Digby-Bell J, Tjir-Li T, Kaser A, Friedman J, MacDonald TT, Bewick GA, Lord GM. Interleukin-22 orchestrates a pathological endoplasmic reticulum stress response transcriptional programme in colonic epithelial cells. Gut. 2020 Mar;69(3):578-590.
  • Temporini C, Colombo R, Calleri E, Tengattini S, Rinaldi F, Massolini G. Chromatographic tools for plant-derived recombinant antibodies purification and characterization. J Pharm Biomed Anal. 2020 Feb 5;179:112920.

Additional Information

Weight 10 oz
Dimensions 7 × 7 × 2 in
Size

100L, 10L, 1L, 50L, 5L
4.8/5 - (127 votes)

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