Inclusion Bodies: Preventing their Formation

Inclusion bodies are often made of denatured aggregates of inactive proteins. Although, the renaturation of inclusion bodies can sometimes lead to the solubilisation and the recovery of active proteins, the process is still very empirical, uncertain and of low efficiency. Several techniques have been developed over the years to prevent the formation of inclusion bodies. […]

Expression of Soluble Proteins in E. coli

Expression of soluble proteins remains a major challenge for biochemists. Although E. coli remains the most straightforward, flexible, fast and inexpensive expression system of heterologous proteins, many proteins accumulate in the cytoplasm of the bacterium in an aggregated misfolded state known as inclusion bodies. Proteins in inclusion bodies are often inactive and attempt to denature […]