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Protocols

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Standard PCR Protocol with Agarose Gel Purification

  • Post author:Molecular Depot
  • Post published:December 1, 2019
  • Post category:Protocols/Science News

Standard PCR Protocol with Agarose Gel Purification This protocol details: How to perform a standard PCR reaction How to extract the target PCR band from an agarose gel How to…

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DNA oligonucleotide 5’-labeling with 32P

  • Post author:Molecular Depot
  • Post published:November 21, 2019
  • Post category:Protocols/Science News

DNA oligonucleotide 5’-labeling with 32P   1- Prepare 5X Kinasing Buffer: 1 M Tris-HCl pH 7.5: 12.5 uL 1 M MgCl2: 2.5 uL 1M DTT: 1.25 uL 10 mM Spermidine:…

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