Standard PCR Protocol with Agarose Gel Purification
Standard PCR Protocol with Agarose Gel Purification This protocol details: How to perform a standard PCR reaction How to extract the target PCR band from an agarose gel How to…
DNA oligonucleotide 5’-labeling with 32P
DNA oligonucleotide 5’-labeling with 32P 1- Prepare 5X Kinasing Buffer: 1 M Tris-HCl pH 7.5: 12.5 uL 1 M MgCl2: 2.5 uL 1M DTT: 1.25 uL 10 mM Spermidine:…