Standard PCR Protocol with Agarose Gel Purification This protocol details: How to perform a standard PCR reaction How to extract the target PCR band from an agarose gel How to digest the target PCR band How to phenol extract the digested PCR band and have it ready for ligation Protocol is courtesy of Molecular Depot.
Category: Protocols
DNA oligonucleotide 5’-labeling with 32P
DNA oligonucleotide 5’-labeling with 32P 1- Prepare 5X Kinasing Buffer: 1 M Tris-HCl pH 7.5: 12.5 uL 1 M MgCl2: 2.5 uL 1M DTT: 1.25 uL 10 mM Spermidine: 2.5 uL 200 mM EDTA: 2.5 uL H2O: 30.75 uL 2- Kinase mix: 5X Kinasing Buffer: 4 uL γ–32P_ATP: x uL (30 to 50 pmoles) […]