Biotin Agarose Suspension (High Binding Capacity)

Product Description

Biotin Agarose Suspension
Catalog number: B2010467
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 2.5 mL
Molecular Weight or Concentration: 4% suspension
Supplied as: Suspension
Appearance: Suspension
Applications: highly pure and high binding capacity agarose beads conjugated to biotin. Capable of binding at least 30 mg of avidin per mL of resin.
Storage: 2-8°C
Keywords: Vitamin H Agarose, biotin conjugated to agarose
Grade: Biotechnology grade. All solid components are highly pure (minimum 95%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
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Balani J, Apple FS, Muthukumar A. Susceptibility of Cardiac Troponin Assays to
Biotin Interference. Am J Clin Pathol. 2019 Apr 2;151(5):486-493.

2: Healy S, Perez-Cadahia B, Jia D, McDonald MK, Davie JR, Gravel RA. Biotin is
not a natural histone modification. Biochim Biophys Acta. 2009 Nov-
Dec;1789(11-12):719-33.

3: Rodgers JT, Patel P, Hennes JL, Bolognia SL, Mascotti DP. Use of biotin-
labeled nucleic acids for protein purification and agarose-based
chemiluminescent electromobility shift assays. Anal Biochem. 2000 Jan
15;277(2):254-9.

4: Lin K, Yan Q, Mitchell A, Funk N, Lu C, Xiao H. A simple method for non-
denaturing purification of biotin-tagged proteins through competitive elution
with free biotin. Biotechniques. 2020 Jan;68(1):41-44.

5: Takakura Y, Sofuku K, Tsunashima M. Tamavidin 2-REV: an engineered tamavidin
with reversible biotin-binding capability. J Biotechnol. 2013 Mar
10;164(1):19-25.
6: Oh-Ishi M, Maeda T. Disease proteomics of high-molecular-mass proteins by
two-dimensional gel electrophoresis with agarose gels in the first dimension
(Agarose 2-DE). J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr
15;849(1-2):211-22.

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