UltraFast Antibody Biotinylation Kit (ug scale, 1 reaction)

Description

UltraFast Antibody and Protein Biotinylation Kit (microgram scale, 1 reaction)
Catalog number: K2010011S
Lot number: Batch Dependent
Expiration Date: Batch dependent
Size: 1 reaction
Storage: 2-8°C
Supplied as: Ready-to-use kit with all required conjugation material

Applications: for custom protein and antibody biotinylation in vitro. 45 min ultrafastand highly efficient protocol. This kit is for the biotinylation of microgram amounts (10-1000 ug). The kit contains all the required materials for 1 biotinylation reaction. Components include NHS-activated Biotin Linkers, biotinylation and storage buffers as well as  size-exlcusion columns for the removal of free unconjugated biotin. Kit has been validated in applications that include ELISA, CLIA, enzymatic tagging and more.

Keywords: Biotinylation Kits, Biotin labelling kit, biotin tagging kit, biotin conjugation kit
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
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for dimerization-dependent protein interactions. FEBS Lett. 2017
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2: Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY.
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in
living human cells by proximity biotinylation. Elife. 2017 Apr 25;6:e24463.

3: Kaewsapsak P, Shechner DM, Mallard W, Rinn JL, Ting AY. Live-cell mapping of
organelle-associated RNAs via proximity biotinylation combined with protein-RNA
crosslinking. Elife. 2017 Dec 14;6:e29224.

4: Beckett D, Kovaleva E, Schatz PJ. A minimal peptide substrate in biotin
holoenzyme synthetase-catalyzed biotinylation. Protein Sci. 1999 Apr;8(4):921-9.

5: Rice GM, Nutiu R, Gampe CM. Direct Chemical Biotinylation of RNA 5′-Ends
Using a Diazo Reagent. Methods Mol Biol. 2019;1870:81-87.

6: Fouda AE, Embogama DM, Ramanayake-Mudiyanselage V, H Pflum MK. Chitosan-
assisted permeabilization of ATP-biotin for live cell kinase-catalyzed
biotinylation. Biotechniques. 2018 Sep;65(3):143-148.

7: Trinh LA, Chong-Morrison V, Sauka-Spengler T. Biotagging, an in vivo
biotinylation approach for cell-type specific subcellular profiling in
zebrafish. Methods. 2018 Nov 1;150:24-31.

8: Varnaitė R, MacNeill SA. Meet the neighbors: Mapping local protein
interactomes by proximity-dependent labeling with BioID. Proteomics. 2016
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