Cholesterol Oxidase from Streptomyces sp.
$195.00
Catalog #: P2010002 – 250 U
Cholesterol oxidase is a flavoprotein involved in the first step of cholesterol catabolism. The enzyme first oxidizes cholesterol to cholest-5-en-3-one then isomerizes it to cholest-4-en-3-one while producing hydrogen peroxide H2O2. Available in a 250 Units and custom bulk sizes.
Live Enquiry about this product via Text/SMS: 1-858-900-3210 (8 am – 8 pm PST)
In stock
Description
Cholesterol Oxidase from Streptomyces sp.
| Catalog # | P2010002 |
| Size | 250 U |
| Molecular Weight | 34 kDa |
| Reaction catalyzed | Cholesterol + O₂ –> Cholest-4-en-3-one + H₂O₂ |
| Unit Definition | One enzymatic unit of cholesterol oxidase converts 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. |
| Specific Activity | 21 U/mg |
| Storage | -20°C. Avoid repeated freeze/thaw cycles. |
| Supplied as | Yellow lyophilized powder (11.9 mg). |
| Application | Assaying cholesterol |
| Keywords | Cholesterol oxidase, cholesterol, flavoprotein. |
About Cholesterol Oxidase
Cholesterol oxidase belongs to the oxidoreductases enzymatic family. This enzyme specifically acts on the CH-OH functional group. The systematic name of the enzymatic class is cholesterol:oxygen oxidoreductase. This enzyme can be also found under the names of cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. The Cholesterol oxidase enzyme plays important roles in the synthesis of bile acids.
References Related to Cholesterol Oxidase from Streptomyces sp.
- Kreit, J., Sampson, N. S. (2009). Cholesterol oxidase: physiological functions. FEBS Journal, 276(23), 6844–6856.
- Vrielink, A., and Ghisla, S. (2009). Cholesterol oxidase: biochemistry and structural features. FEBS Journal, 276(23), 6826–6843.
- Vrielink, A. (2010). Cholesterol Oxidase: Structure and Function. Cholesterol Binding and Cholesterol Transport Proteins: Subcellular Biochemistry, 137–158.
- Niwas, R., Singh, V., Singh, R., Pant, G., Mitra, K., and Tripathi, C. K. M. (2014). Cholesterol oxidase production from entrapped cells ofStreptomycessp. Journal of Basic Microbiology, 54(11), 1233–1239.
- Pollegioni, L. (2009). Cholesterol oxidase: a model flavoprotein oxidase and a biotechnological tool. FEBS Journal, 276(23), 6825–6825.
- Olivares-Rubio HF, Salazar-Coria L, Romero-López JP, Domínguez-López ML, García-Latorre EA, Vega-López A. Fatty acid metabolism and brain mitochondrial performance of juvenile Nile tilapia (Oreochromis niloticus) exposed to the water-accommodated fraction of Maya crude oil. Ecotoxicol Environ Saf. 2020 Apr 14;197:110624.
- El-Naggar NE, El-Shweihy NM. Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94. BMC Microbiol. 2020 Apr 10;20(1):86.
Ordering Information
1- Order online using our website’s automated shopping cart and checkout system (available 24/7).
2- Order by email: info@moleculardepot.com (we accept electronic PO, available 24/7).
3- Order by Fax: 1-858-437-9884 (available 24/7).
4- Order by phone: 1-858-900-3210 (6 am – 8 pm PST).
5- Order by Text/SMS: 1-858-900-3210 (6 am – 8 pm PST).
