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Cholesterol Oxidase

$450.00 $195.00

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Cholesterol Oxidase

$450.00 $195.00

Catalog #: P2010002 – 250 U

Cholesterol oxidase is a flavoprotein involved in the first step of cholesterol catabolism. The enzyme first oxidizes cholesterol to cholest-5-en-3-one then isomerizes it to cholest-4-en-3-one while producing hydrogen peroxide H2O2. Available in a 250 Units and custom bulk sizes.

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SKU: P2010002 Category: Tag:

Description

Cholesterol Oxidase from Streptomyces sp.

Product Data Sheet
Catalog # P2010002
Size 250 U
Molecular Weight 34 kDa
Reaction catalyzed Cholesterol + O₂ –> Cholest-4-en-3-one + H₂O₂
Unit Definition One enzymatic unit of cholesterol oxidase converts 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C.
Specific Activity 21 U/mg
Storage -20°C. Avoid repeated freeze/thaw cycles.
Supplied as Yellow lyophilized powder (11.9 mg).
Application Assaying cholesterol
Keywords Cholesterol oxidase, cholesterol, flavoprotein.

References

  • Kreit, J., Sampson, N. S. (2009). Cholesterol oxidase: physiological functions. FEBS Journal, 276(23), 6844–6856.
  • Vrielink, A., and Ghisla, S. (2009). Cholesterol oxidase: biochemistry and structural features. FEBS Journal, 276(23), 6826–6843.
  • Vrielink, A. (2010). Cholesterol Oxidase: Structure and Function. Cholesterol Binding and Cholesterol Transport Proteins: Subcellular Biochemistry, 137–158.
  • Niwas, R., Singh, V., Singh, R., Pant, G., Mitra, K., and Tripathi, C. K. M. (2014). Cholesterol oxidase production from entrapped cells ofStreptomycessp. Journal of Basic Microbiology, 54(11), 1233–1239.
  • Pollegioni, L. (2009). Cholesterol oxidase: a model flavoprotein oxidase and a biotechnological tool. FEBS Journal, 276(23), 6825–6825.

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