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Cholesterol Oxidase from Streptomyces sp.

$450.00 $195.00

Catalog #: P2010002 – 250 U

Cholesterol oxidase is a flavoprotein involved in the first step of cholesterol catabolism. The enzyme first oxidizes cholesterol to cholest-5-en-3-one then isomerizes it to cholest-4-en-3-one while producing hydrogen peroxide H2O2. Available in a 250 Units and custom bulk sizes.

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Description

Cholesterol Oxidase from Streptomyces sp.

Product Data Sheet
Catalog # P2010002
Size 250 U
Molecular Weight 34 kDa
Reaction catalyzed Cholesterol + O₂ –> Cholest-4-en-3-one + H₂O₂
Unit Definition One enzymatic unit of cholesterol oxidase converts 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C.
Specific Activity 21 U/mg
Storage -20°C. Avoid repeated freeze/thaw cycles.
Supplied as Yellow lyophilized powder (11.9 mg).
Application Assaying cholesterol
Keywords Cholesterol oxidase, cholesterol, flavoprotein.

 

About Cholesterol Oxidase

Cholesterol oxidase belongs to the oxidoreductases enzymatic family. This enzyme specifically acts on the CH-OH functional group. The systematic name of the enzymatic class is cholesterol:oxygen oxidoreductase. This enzyme can be also found under the names of  cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. The Cholesterol oxidase enzyme plays important roles in the synthesis of bile acids.

References Related to  Cholesterol Oxidase from Streptomyces sp.

  • Kreit, J., Sampson, N. S. (2009). Cholesterol oxidase: physiological functions. FEBS Journal, 276(23), 6844–6856.
  • Vrielink, A., and Ghisla, S. (2009). Cholesterol oxidase: biochemistry and structural features. FEBS Journal, 276(23), 6826–6843.
  • Vrielink, A. (2010). Cholesterol Oxidase: Structure and Function. Cholesterol Binding and Cholesterol Transport Proteins: Subcellular Biochemistry, 137–158.
  • Niwas, R., Singh, V., Singh, R., Pant, G., Mitra, K., and Tripathi, C. K. M. (2014). Cholesterol oxidase production from entrapped cells ofStreptomycessp. Journal of Basic Microbiology, 54(11), 1233–1239.
  • Pollegioni, L. (2009). Cholesterol oxidase: a model flavoprotein oxidase and a biotechnological tool. FEBS Journal, 276(23), 6825–6825.
  • Olivares-Rubio HF, Salazar-Coria L, Romero-López JP, Domínguez-López ML, García-Latorre EA, Vega-López A. Fatty acid metabolism and brain mitochondrial performance of juvenile Nile tilapia (Oreochromis niloticus) exposed to the water-accommodated fraction of Maya crude oil. Ecotoxicol Environ Saf. 2020 Apr 14;197:110624.
  • El-Naggar NE, El-Shweihy NM. Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94. BMC Microbiol. 2020 Apr 10;20(1):86.

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