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His Tag Antibody (Mouse Monoclonal)

$595.00 $395.00

Catalog: A2010011 – 0.25 mg

The hexahistidine epitope (His)6 is one of the most used epitopes for tagging proteins and purifying them on (His)6-binding resins such as Ni-NTA and Co-NTA. This antibody specifically targets the (His)6 tag of proteins and allows their detection by ELISA, Western Blot and Immuno-precipitation. Bulk pricing and biotinylation services are available.

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SKU: A2010011 Category: Tags: ,

Description

Antibody to (His)6 Tag Monoclonal.

Product Data Sheet
Catalog # A2010011
Size 0.25 mg
Other Names Histidine tag antibody, hexahistidine tag antibody, His6 antibody, polyhistidine tag
Host Mouse
Concentration 2 mg/mL
Purity Affinity purified of protein G (> 95%)
Storage 2-8°C (short term). -20°C (long term). Avoid repeated freeze-thaw.
Supplied as Liquid solution
Buffer PBS1x, pH 7.4 + 0.05% sodium azide
Keywords Histidine tag, antibody

 

References

  • Construction of Escherichia coli BL21/A-53 producing histidine-tagged carboxymethylcellulase and comparison of its characteristics with CMCase without histidine-tag. Kang DU, Lee YS, Lee JW. Prep Biochem Biotechnol. 2019;49(2):167-175.
  • Activity enhancement of CotA laccase by hydrophilic engineering, histidinetag optimization and static culture. Li L, Xie T, Liu Z, Feng H, Wang G. Protein Eng Des Sel. 2018 Jan 1;31(1):1-5.
  • Binding Characteristics of IFN-alpha Subvariants to IFNAR2-EC and Influence of the 6-HistidineTag. Schmeisser H, Kontsek P, Esposito D, Gillette W, Schreiber G, Zoon KC. J Interferon Cytokine Res. 2006 Dec;26(12):866-76.
  • Tailoring of recombinant FDH: effect of histidinetag location on solubility and catalytic properties of Chaetomium thermophilum formate dehydrogenase (CtFDH). Esen H, Alpdağtaş S, Mervan Çakar M, Binay B. Prep Biochem Biotechnol. 2019;49(5):529-534.
  • Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes. Kinoshita-Kikuta E, Kusamoto H, Ono S, Akayama K, Eguchi Y, Igarashi M, Okajima T, Utsumi R, Kinoshita E, Koike T. Electrophoresis. 2019 Nov;40(22):3005-3013.
  • Effect of histidine-tag and R457H and E580Q mutations on catalytic activity of recombinant human cytochrome P450 oxidoreductase. Niwa T, Minami K, Katagiri M. Drug Metab Pharmacokinet. 2012;27(1):150-4.
  • Effect of C-terminal His-tag and purification routine on the activity and structure of the metalloenzyme, l-alanyl-d-glutamate peptidase of the bacteriophage T5. Kutyshenko VP, Mikoulinskaia GV, Chernyshov SV, Yegorov AY, Prokhorov DA, Uversky VN. Int J Biol Macromol. 2019 Mar 1;124:810-818.
  • A modified His-tag vector for the production of recombinant protein kinases. Lomas-Lopez R, Cozzone AJ, Duclos B. Anal Biochem. 2008 Jun 15;377(2):272-3.

Material Safety Data Sheet