TR-FRET Detection Buffer

Product Description

TR-FRET Detection Buffer
Catalog number: B2011224
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: 2-8 °C
Keywords: TR-FRET Detection Buffer for Europium Labeled Antibodies
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Degorce F, Card A, Soh S, Trinquet E, Knapik GP, Xie B. HTRF: A technology tailored for drug discovery – a review of theoretical aspects and recent applications Curr Chem Genomics. 2009 May 28;3:22-32.
2: Lee S, Abed DA, Beamer LJ, Hu L. Development of a Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Assay for the Inhibition of Keap1-Nrf2 Protein-Protein Interaction SLAS Discov. 2021 Jan;26(1):100-112.
3: Ardecky R, Madiraju C, Matsuzawa SI, Zou J, Ganji S, Pass I, Ngo TA, Pinkerton AB, Sergienko E, Su Y, Stonich D, Mangravita-Novo A, Vicchiarelli M, McAnally D, Smith LH, Diwan J, Chung TDY, Matsuzawa Y, Wimer C, Diaz PW, Reed JC. Selective UBC 13 Inhibitors 2012 Apr 16 [updated 2014 May 13]. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010–.
4: Hancock MK, Hermanson SB, Dolman NJ. A quantitative TR-FRET plate reader immunoassay for measuring autophagy Autophagy. 2012 Aug;8(8):1227-44.
5: Rusanen J, Kareinen L, Szirovicza L, Uğurlu H, Levanov L, Jääskeläinen A, Ahava M, Kurkela S, Saksela K, Hedman K, Vapalahti O, Hepojoki J. A Generic, Scalable, and Rapid Time-Resolved Förster Resonance Energy Transfer-Based Assay for Antigen Detection-SARS-CoV-2 as a Proof of Concept mBio. 2021 May 18;12(3):e00902-21.
6: Wang M, Ye H, You L, Chen X. A Supramolecular Sensor Array Using Lanthanide-Doped Nanoparticles for Sensitive Detection of Glyphosate and Proteins ACS Appl Mater Interfaces. 2016 Jan 13;8(1):574-81.
7: Atanasov AG, Wang JN, Gu SP, Bu J, Kramer MP, Baumgartner L, Fakhrudin N, Ladurner A, Malainer C, Vuorinen A, Noha SM, Schwaiger S, Rollinger JM, Schuster D, Stuppner H, Dirsch VM, Heiss EH. Honokiol: a non-adipogenic PPARγ agonist from nature Biochim Biophys Acta. 2013 Oct;1830(10):4813-9.
8: Sharlow ER, Leimgruber S, Yellow-Duke A, Barrett R, Wang QJ, Lazo JS. Development, validation and implementation of immobilized metal affinity for phosphochemicals (IMAP)-based high-throughput screening assays for low-molecular-weight compound libraries Nat Protoc. 2008;3(8):1350-63.
9: Peña I, Domínguez JM. Thermally denatured BSA, a surrogate additive to replace BSA in buffers for high-throughput screening J Biomol Screen. 2010 Dec;15(10):1281-6.
10: Mohamed TM, Zakeri SA, Baudoin F, Wolf M, Oceandy D, Cartwright EJ, Gul S, Neyses L. Optimisation and validation of a high throughput screening compatible assay to identify inhibitors of the plasma membrane calcium ATPase pump–a novel therapeutic target for contraception and malaria J Pharm Pharm Sci. 2013;16(2):217-30.

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