Tris Buffer pH 7.0 Solution (1M, Biotechnology Grade)

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1M Tris Buffer pH 7.0
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Tris Buffer pH 7.0 Solution (1M, Biotechnology Grade)

Original price was: $795.00.Current price is: $395.00.

Catalog Number: B2010021 (50 mL)
Tris Buffer pH 7.0 (1M, Biotechnology Grade) is a highly pure, sterile-filtered buffering solution formulated with ultrapure deionized water and used to maintain a stable pH environment in biochemistry and molecular biology. It is essential for various applications including protein purification, cell lysis, nucleic acid extraction, and enzyme assays. This ready-to-use solution has a temperature-dependent pH, typically calibrated at 25°C, and is verified to be free of DNase, RNase, and protease.

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Product Description

4.9/5 - (9 votes)

1M Tris Buffer pH 7.0

Catalog number: B2010021
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume: 50 mL
Formula NH2C(CH2OH)3
MW 121.14
Other names 2-Amino-2-(hydroxymethyl)–1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Application: Buffer and solution formulation, reagent formulation
Shelf-Life Two years from date of manufacture
Storage: Room temperature
Keywords: tris solution, tris buffer, tris buffer neutral, tris-HCl buffer, tris-Cl, tris solution
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Iwai R, Haruki R, Nemoto Y, Nakayama Y. Enhanced transfection efficiency of
poly(N,N-dimethylaminoethyl methacrylate)-based deposition transfection by
combination with tris(hydroxymethyl)aminomethane. Bioconjug Chem. 2013 Feb
20;24(2):159-66.2: Gumbhir K, Mason WD. High-performance liquid chromatographic method for the
determination of tris(hydroxymethyl)aminomethane (tromethamine) in human plasma. J Chromatogr. 1992 Nov 27;583(1):99-104.

3: Bhattacharya A, Chattopadhyay B, Chakraborty S, Roy BN, Singh GP, Godbole HM, Rananaware UB, Mukherjee AK. Tris(hydroxymethyl) aminomethane salt of ramipril: synthesis, structural characterization from X-ray powder diffraction and
stability studies. J Pharm Biomed Anal. 2012 Nov;70:280-7.

4: Taha M, Lee MJ. Interactions of TRIS [tris(hydroxymethyl)aminomethane] and
related buffers with peptide backbone: thermodynamic characterization. Phys Chem
Chem Phys. 2010 Oct 21;12(39):12840-50.

5: Blanke SR, Blanke RV. The Schotten-Baumann reaction as an aid to the analysis
of polar compounds: application to the determination of
tris(hydroxymethyl)aminomethane (THAM). J Anal Toxicol. 1984 Sep-Oct;8(5):231-3.

6: Burcham PC, Fontaine FR, Petersen DR, Pyke SM. Reactivity with
Tris(hydroxymethyl)aminomethane confounds immunodetection of acrolein-adducted proteins. Chem Res Toxicol. 2003 Oct;16(10):1196-201.

7: Shiraishi H, Kataoka M, Morita Y, Umemoto J. Interactions of hydroxyl
radicals with tris (hydroxymethyl) aminomethane and Good’s buffers containing
hydroxymethyl or hydroxyethyl residues produce formaldehyde. Free Radic Res
Commun. 1993;19(5):315-21.

8: Harsha SS, Grischkowsky D. Terahertz (far-infrared) characterization of
tris(hydroxymethyl)aminomethane using high-resolution waveguide THz-TDS. J Phys
Chem A. 2010 Mar 18;114(10):3489-94.
Products Realted to Tris Buffer pH 7 Biochemical Buffers and Solutions

Additional Information

Weight 2.6 oz
Dimensions 3.3 × 1.2 × 1.2 in
4.9/5 - (9 votes)

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