ELISA Blocking Buffer (10X Solution)

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ELISA Blocking Buffer
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ELISA Blocking Buffer (10X Solution)

Original price was: $550.00.Current price is: $295.00.

Catalog #: B2010010 (100 mL, 10X)
Concentrated solution (10X) of ELISA Blocking Buffer that must be diluted with di-water to a 1X working solution before use. This buffer has been extensively validated for use in ELISA applications by coating various proteins, protein-conjugates and antibodies. This buffer can be used during the production of ELISA coated microplates.

Large bulk volumes available at discounted prices (inquire here).

In stock

SKU: B2010010 Categories: , Tag:

Product Description

4.9/5 - (14 votes)

ELISA Blocking Buffer (10X)

Catalog number: B2010010
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume: 100 mL
Appearance: Clear to slightly white Solution
Application: ELISA
Shelf-Life 3 years
Storage: Room temperature
Keywords: Elisa blocking protocol, ELISA blocking buffer, blocking Elisa plates, blocking buffer Elisa.

ELISA Coating/Washing/Blocking Protocol

Detailed protocol is provided.

References

  • 1: Enzyme immunoassay and enzyme-linked immunosorbent assay. Gan SD, Patel KR. J Invest Dermatol. 2013 Sep;133(9):e12.
  • 2: Enzyme-linkedimmunosorbentassay (ELISA). Reen DJ. Methods Mol Biol. 1994;32:461-6.
  • 3: Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection. Arya SK, Estrela P. Sensors (Basel). 2018 Jun 22;18(7):2010.
  • 4: The application of immunoassay techniques, including enzyme-linkedimmunosorbentassay (ELISA), to snake venom research. Theakston RD. Toxicon. 1983;21(3):341-52.
  • 5: Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis. Brasil PE, Castro R, Castro Ld. Mem Inst Oswaldo Cruz. 2016 Jan;111(1):1-19.
  • 6: Lequin RM. Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay
    (ELISA). Clin Chem. 2005 Dec;51(12):2415-8.
  • 7: Aydin S. A short history, principles, and types of ELISA, and our laboratory
    experience with peptide/protein analyses using ELISA. Peptides. 2015
    Oct;72:4-15.
  • 8: Eble JA. Titration ELISA as a Method to Determine the Dissociation Constant
    of Receptor Ligand Interaction. J Vis Exp. 2018 Feb 15;(132):57334.
  • 9: Pang B, Zhao C, Li L, Song X, Xu K, Wang J, Liu Y, Fu K, Bao H, Song D, Meng
    X, Qu X, Zhang Z, Li J. Development of a low-cost paper-based ELISA method for
    rapid Escherichia coli O157:H7 detection. Anal Biochem. 2018 Feb 1;542:58-62.
  • 10: Major M, Law M. Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA.
    Methods Mol Biol. 2019;1911:421-432. doi: 10.1007/978-1-4939-8976-8_28.

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Additional Information

Weight 5.5 oz
Dimensions 5 × 3 × 3 in
4.9/5 - (14 votes)

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