E. coli Glycerol Kinase

Product Description

E. coli Glycerol Kinase
Catalog number: B2015196
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 ug
Molecular Weight or Concentration: 58.6 kDa (525 aa)
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -20° C
Keywords: Glycerol Kinase, ATP:Glycerol 3-Phosphotransferase, Glycerokinase, GK, glpK
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Thorner JW. Glycerol kinase Methods Enzymol. 1975;42:148-56.
2: Voegele RT, Sweet GD, Boos W. Glycerol kinase of Escherichia coli is activated by interaction with the glycerol facilitator J Bacteriol. 1993 Feb;175(4):1087-94.
3: Soellner S, Rahnert M, Siemann-Herzberg M, Takors R, Altenbuchner J. Evolution of pyruvate kinase-deficient Escherichia coli mutants enables glycerol-based cell growth and succinate production J Appl Microbiol. 2013 Dec;115(6):1368-78.
4: Pettigrew DW. Amino acid substitutions in the sugar kinase/hsp70/actin superfamily conserved ATPase core of E. coli glycerol kinase modulate allosteric ligand affinity but do not alter allosteric coupling Arch Biochem Biophys. 2009 Jan 15;481(2):151-6.
5: Zwaig N, Kistler WS, Lin EC. Glycerol kinase, the pacemaker for the dissimilation of glycerol in Escherichia coli J Bacteriol. 1970 Jun;102(3):753-9.
6: Tang Y, Shi Y, Jia B, Zhang Y, Wang Q. Evolution and function analysis of glycerol kinase GlpK in Pseudomonasaeruginosa Biochem Biophys Res Commun. 2023 Feb 19;645:30-39.
7: Escobedo-Hinojosa W, Wissner JL, Hauer B. A real-time (31)P-NMR-based approach for the assessment of glycerol kinase catalyzed monophosphorylations MethodsX. 2021 Feb 19;8:101285.
8: Guo JY, Hu KL, Bi CH, Li QY, Zhang XL. Construction of an alternative glycerol-utilization pathway for improved β-carotene production in Escherichia coli J Ind Microbiol Biotechnol. 2018 Aug;45(8):697-705.
9: Wang G, Wang M, Liu L, Hui X, Wang B, Ma K, Yang X. Improvement of the catalytic performance of glycerol kinase from Bacillus subtilis by chromosomal site-directed mutagenesis Biotechnol Lett. 2022 Sep;44(9):1051-1061.
10: Zhu H, Yi X, Liu Y, Hu H, Wood TK, Zhang X. Production of acetol from glycerol using engineered Escherichia coli Bioresour Technol. 2013 Dec;149:238-43.

Products Related to E. coli Glycerol Kinase can be found at Enzymes