BirA Protein

Product Description

BirA Protein
Catalog number: B2016680
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 µg
Molecular Weight or Concentration: 38 kDa
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -80°C
Keywords: Recombinant BirA Protein, His-Tag
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Ramanathan M, Majzoub K, Rao DS, Neela PH, Zarnegar BJ, Mondal S, Roth JG, Gai H, Kovalski JR, Siprashvili Z, Palmer TD, Carette JE, Khavari PA. RNA-protein interaction detection in living cells Nat Methods. 2018 Mar;15(3):207-212.
2: Roux KJ, Kim DI, Burke B, May DG. BioID: A Screen for Protein-Protein Interactions Curr Protoc Protein Sci. 2018 Feb 21;91:19.23.1-19.23.15.
3: Wang Z, Li Z, Su T, Han X, Hou Z, Zheng Y, Liu J, Xu J, Yang J, Liu H. BirA*-protein A fusion protein based BioEnhancer amplifies western blot immunosignal Electrophoresis. 2021 Mar;42(6):793-799.
4: Roux KJ, Kim DI, Raida M, Burke B. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells J Cell Biol. 2012 Mar 19;196(6):801-10.
5: Fairhead M, Howarth M. Site-specific biotinylation of purified proteins using BirA Methods Mol Biol. 2015;1266:171-84.
6: Habel JE. Biotin Proximity Labeling for Protein-Protein Interaction Discovery: The BioID Method Methods Mol Biol. 2021;2261:357-379.
7: Larochelle M, Bergeron D, Arcand B, Bachand F. Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast J Cell Sci. 2019 May 31;132(11):jcs232249.
8: Kido K, Yamanaka S, Nakano S, Motani K, Shinohara S, Nozawa A, Kosako H, Ito S, Sawasaki T. AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions Elife. 2020 May 11;9:e54983.
9: Zhang Y, Li Y, Yang X, Wen Z, Nagalakshmi U, Dinesh-Kumar SP. TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks J Vis Exp. 2020 May 17;(159):10.3791/60728.
10: Li Y, Sousa R. Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation Protein Expr Purif. 2012 Mar;82(1):162-7.

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