Horseradish Peroxidase Biotinylated
$175.00
Catalog #: P2010010 – 1 mg
Horseradish Peroxidase (HRP) is one of the most used enzyme in biochemical applications. It catalyzes the oxidation of a large variety of substrates using Hydrogen Peroxide as oxidizing agent. Biotinylated HRP can used to amplify the signal of certain immunoassays.
Live Enquiry about this product via Text/SMS: 1-858-900-3210 (8 am – 8 pm PST)
In stock
Description
Horseradish Peroxidase Biotinylated
Catalog number: | P2010010 |
Lot number: | Batch Dependent |
Expiration date: | Batch Dependent |
Package Size: | 1 mg |
Specific Activity: | 350 U/mg |
Unit Definition: | Amount of biotinylated HRP needed to produce 1 μmole of purpurogallin from pyrogallol in 20 seconds at 20°C in reaction buffer: 8 mM hydrogen peroxide, 14 mM potassium phosphate, 0.5% pyrogallol, pH 6.0. |
Biotin Incorporation: | 1.3 moles biotin/mole HRP (HABA assay) |
Appearance: | Lyophilized powder |
Storage: | -20°C or lower. Avoid repeated freeze-thaw. |
Purity: | >95%. |
Recommended Buffer: | 10 mM citrate, pH 6.0 |
References Related to Horseradish Peroxidase Biotinylated
- False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences. Clin Chem Lab Med. 2017 May 1;55(6):780-788.
- Characterization of the scope and magnitude of biotin interference in susceptible Roche Elecsys competitive and sandwich immunoassays.Ann Clin Biochem. 2018 Mar;55(2):205-215.
- Depletion of biotin using streptavidin-coated microparticles: a validated solution to the problem of biotin interference in streptavidin-biotin immunoassays.Ann Clin Biochem. 2018 Mar;55(2):216-226.
- Current good manufacturing practices-compliant manufacture and measurement of biotin-labeled red blood cells.Cytotherapy. 2019 Jul;21(7):793-800.
- Characterization of biotin interference in 21 Vitros 5600 immunoassays and risk mitigation for patient safety at a large academic medical center.Clin Biochem. 2020 Jan;75:53-61.
- Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation.Anal Biochem. 2018 Sep 15;557:46-58.
- Biotin accounts for less than half of all biotin and biotin metabolites in the cerebrospinal fluid of children.Am J Clin Nutr. 2008 Nov;88(5):1291-6.
- Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques.Methods Mol Biol. 2008;418:111-28.
Ordering Information
1- Order online using our website’s automated shopping cart and checkout system (available 24/7).
2- Order by email: info@moleculardepot.com (we accept electronic PO, available 24/7).
3- Order by Fax: 1-858-437-9884 (available 24/7).
4- Order by phone: 1-858-900-3210 (6 am – 8 pm PST).
5- Order by Text/SMS: 1-858-900-3210 (6 am – 8 pm PST).