Horseradish Peroxidase Biotinylated


Catalog #: P2010010 – 1 mg

Horseradish Peroxidase (HRP) is one of the most used enzyme in biochemical applications. It catalyzes the oxidation of a large variety of substrates using Hydrogen Peroxide as oxidizing agent. Biotinylated HRP can used to amplify the signal of certain immunoassays.

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Horseradish Peroxidase Biotinylated

Catalog number: P2010010
Lot number: Batch Dependent
Expiration date: Batch Dependent
Package Size: 1 mg
Specific Activity: 350 U/mg
Unit Definition: Amount of biotinylated HRP needed to produce 1 μmole of purpurogallin from pyrogallol in 20 seconds at 20°C in reaction buffer: 8 mM hydrogen peroxide, 14 mM potassium phosphate, 0.5% pyrogallol, pH 6.0.
Biotin Incorporation: 1.3 moles biotin/mole HRP (HABA assay)
Appearance: Lyophilized powder
Storage: -20°C or lower. Avoid repeated freeze-thaw.
Purity: >95%.
Recommended Buffer: 10 mM citrate, pH 6.0


References Related to Horseradish Peroxidase Biotinylated

  • False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences. Piketty ML, Polak M, Flechtner I, Gonzales-Briceño L, Souberbielle JC. Clin Chem Lab Med. 2017 May 1;55(6):780-788.
  • Characterization of the scope and magnitude of biotin interference in susceptible Roche Elecsys competitive and sandwich immunoassays. Trambas C, Lu Z, Yen T, Sikaris K. Ann Clin Biochem. 2018 Mar;55(2):205-215.
  • Depletion of biotin using streptavidin-coated microparticles: a validated solution to the problem of biotin interference in streptavidin-biotin immunoassays. Trambas C, Lu Z, Yen T, Sikaris K. Ann Clin Biochem. 2018 Mar;55(2):216-226.
  • Current good manufacturing practices-compliant manufacture and measurement of biotin-labeled red blood cells. Donnenberg AD, Kanias T, Triulzi DJ, Dennis CJ, Moore LR, Meyer EM, Sinchar D, Kiss JE, Normolle DP, Gladwin MT. Cytotherapy. 2019 Jul;21(7):793-800.
  • Characterization of biotin interference in 21 Vitros 5600 immunoassays and risk mitigation for patient safety at a large academic medical center. Stieglitz HM, Korpi-Steiner N. Clin Biochem. 2020 Jan;75:53-61.
  • Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation. Muraoka A, Matsuura Y, Naitow H, Ihara M, Kunishima N. Anal Biochem. 2018 Sep 15;557:46-58.
  • Biotin accounts for less than half of all biotin and biotin metabolites in the cerebrospinal fluid of children. Bogusiewicz A, Stratton SL, Ellison DA, Mock DM. Am J Clin Nutr. 2008 Nov;88(5):1291-6.
  • Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques. McKay BE, Molineux ML, Turner RW. Methods Mol Biol. 2008;418:111-28.

Additional information

Weight 32 oz
Dimensions 8 × 6.75 × 5.5 in

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