Horseradish Peroxidase Biotinylated
$295.00
Catalog #: P2010010 – 1 mg
Horseradish Peroxidase (HRP) is one of the most used enzyme in biochemical applications. It catalyzes the oxidation of a large variety of substrates using Hydrogen Peroxide as oxidizing agent. Biotinylated HRP can used to amplify the signal of certain immunoassays.
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In stock
Description
Horseradish Peroxidase Biotinylated
| Catalog number: | P2010010 |
| Lot number: | Batch Dependent |
| Expiration date: | Batch Dependent |
| Package Size: | 1 mg |
| Specific Activity: | 350 U/mg |
| Unit Definition: | Amount of biotinylated HRP needed to produce 1 μmole of purpurogallin from pyrogallol in 20 seconds at 20°C in reaction buffer: 8 mM hydrogen peroxide, 14 mM potassium phosphate, 0.5% pyrogallol, pH 6.0. |
| Biotin Incorporation: | 1.3 moles biotin/mole HRP (HABA assay) |
| Appearance: | Lyophilized powder |
| Storage: | -20°C or lower. Avoid repeated freeze-thaw. |
| Purity: | >95%. |
| Recommended Buffer: | 10 mM citrate, pH 6.0 |
References Related to Horseradish Peroxidase Biotinylated
- False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences. Clin Chem Lab Med. 2017 May 1;55(6):780-788.
- Characterization of the scope and magnitude of biotin interference in susceptible Roche Elecsys competitive and sandwich immunoassays. Ann Clin Biochem. 2018 Mar;55(2):205-215.
- Depletion of biotin using streptavidin-coated microparticles: a validated solution to the problem of biotin interference in streptavidin-biotin immunoassays. Ann Clin Biochem. 2018 Mar;55(2):216-226.
- Current good manufacturing practices-compliant manufacture and measurement of biotin-labeled red blood cells. Cytotherapy. 2019 Jul;21(7):793-800.
- Characterization of biotin interference in 21 Vitros 5600 immunoassays and risk mitigation for patient safety at a large academic medical center. Clin Biochem. 2020 Jan;75:53-61.
- Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation. Anal Biochem. 2018 Sep 15;557:46-58.
- Biotin accounts for less than half of all biotin and biotin metabolites in the cerebrospinal fluid of children. Am J Clin Nutr. 2008 Nov;88(5):1291-6.
- Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques. Methods Mol Biol. 2008;418:111-28.
Additional information
| Weight | 32 oz |
|---|---|
| Dimensions | 8 × 6.75 × 5.5 in |
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