Biotinylated Mononucleosomes (H1.2)

Product Description

Biotinylated Mononucleosomes (H1.2)
Catalog number: B2016651
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 µg
Molecular Weight or Concentration: histone octamer and histone H1.2 are 108 kDa and 21.4 kDa, respectively
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -80°C
Keywords: Recombinant Mononucleosomes (H1.2) – Biotin
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Gerodimos CA, Watanabe G, Lieber MR. Nonhomologous DNA end joining of nucleosomal substrates in a purified system DNA Repair (Amst). 2021 Oct;106:103193.
2: Laflamme L, Guillemette B, Gaudreau L. An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro Methods Mol Biol. 2017;1528:19-37.
3: Fang H, Wei S, Lee TH, Hayes JJ. Chromatin structure-dependent conformations of the H1 CTD Nucleic Acids Res. 2016 Nov 2;44(19):9131-9141.
4: Changolkar LN, Pehrson JR. Reconstitution of nucleosomes with histone macroH2A1.2 Biochemistry. 2002 Jan 8;41(1):179-84.
5: Aizawa M, Sugimoto N, Watanabe S, Yoshida K, Fujita M. Nucleosome assembly and disassembly activity of GRWD1, a novel Cdt1-binding protein that promotes pre-replication complex formation Biochim Biophys Acta. 2016 Nov;1863(11):2739-2748.
6: Kepert JF, Tóth KF, Caudron M, Mücke N, Langowski J, Rippe K. Conformation of reconstituted mononucleosomes and effect of linker histone H1 binding studied by scanning force microscopy Biophys J. 2003 Dec;85(6):4012-22.
7: Bennett L, Madders ECET, Parsons JL. HECTD1 promotes base excision repair in nucleosomes through chromatin remodelling Nucleic Acids Res. 2020 Feb 20;48(3):1301-1313.
8: Le DD, Cortesi AT, Myers SA, Burlingame AL, Fujimori DG. Site-specific and regiospecific installation of methylarginine analogues into recombinant histones and insights into effector protein binding J Am Chem Soc. 2013 Feb 27;135(8):2879-82.
9: Ho KK, Zhang H, Golden BL, Ogas J. PICKLE is a CHD subfamily II ATP-dependent chromatin remodeling factor Biochim Biophys Acta. 2013 Feb;1829(2):199-210.
10: Ruhl DD, Jin J, Cai Y, Swanson S, Florens L, Washburn MP, Conaway RC, Conaway JW, Chrivia JC. Purification of a human SRCAP complex that remodels chromatin by incorporating the histone variant H2A.Z into nucleosomes Biochemistry. 2006 May 2;45(17):5671-7.

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