TEV Protease Conjugated to Biotin

Product Description

TEV Protease Conjugated to Biotin
Catalog number: B2014415
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 2 kU
Molecular Weight or Concentration: N/A
Supplied as: N/A
Applications: molecular tool for various biochemical applications
Storage: −20°C
Keywords: TEV Protease, Biotin tagged
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Li Y, Sousa R. Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation Protein Expr Purif. 2012 Mar;82(1):162-7.
2: Verma V, Kaur C, Grover P, Gupta A, Chaudhary VK. Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection PLoS One. 2018 Jan 23;13(1):e0191315.
3: Norris JL, Patel T, Dasari AKR, Cope TA, Lim KH, Hughes RM. Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles J Biotechnol. 2020 Oct 10;322:1-9.
4: Bailey D, Urena L, Thorne L, Goodfellow I. Identification of protein interacting partners using tandem affinity purification J Vis Exp. 2012 Feb 25;(60):3643.
5: Embaby AM, Schoffelen S, Kofoed C, Meldal M, Diness F. Rational Tuning of Fluorobenzene Probes for Cysteine-Selective Protein Modification Angew Chem Int Ed Engl. 2018 Jul 2;57(27):8022-8026.
6: Li Y, Sousa R. Novel system for in vivo biotinylation and its application to crab antimicrobial protein scygonadin Biotechnol Lett. 2012 Sep;34(9):1629-35.
7: Nagy SK, Kllai BM, Andrs J, Mszros T. A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression BMC Biotechnol. 2020 Mar 14;20(1):17.
8: Klein S, Stern D, Seeber F. Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays BMC Biotechnol. 2020 Oct 6;20(1):53.
9: Ioannou M, Papageorgiou DN, Ogryzko V, Strouboulis J. Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase BMC Res Notes. 2018 Jun 14;11(1):390.
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