Immobilized Ficin Agarose Resin

Product Description

Immobilized Ficin Agarose Resin
Catalog number: B2015565
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 2 mL
Molecular Weight or Concentration: N/A
Supplied as: Suspension
Applications: a molecular tool for various biochemical applications
Storage: 2-8°C
Keywords: Immobilized Ficin Agarose Resin
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Siar EH, Morellon-Sterling R, Zidoune MN, Fernandez-Lafuente R. Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts Int J Biol Macromol. 2020 Feb 1;144:419-426.
2: Siar EH, Morellon-Sterling R, Zidoune MN, Fernandez-Lafuente R. Amination of ficin extract to improve its immobilization on glyoxyl-agarose: Improved stability and activity versus casein Int J Biol Macromol. 2019 Jul 15;133:412-419.
3: Morellon-Sterling R, Bolivar JM, Fernandez-Lafuente R. Switch off/switch on of a cysteinyl protease as a way to preserve the active catalytic group by modification with a reversible covalent thiol modifier: Immobilization of ficin on vinyl-sulfone activated supports Int J Biol Macromol. 2022 Nov 1;220:1155-1162.
4: Sattari F, Rigi G, Ghaedmohammadi S. The first report on molecular cloning, functional expression, purification, and statistical optimization of Escherichia coli-derived recombinant Ficin from Iranian fig tree (Ficus carica cv.Sabz) Int J Biol Macromol. 2020 Dec 15;165(Pt B):2126-2135.
5: Baeyens-Volant D, Matagne A, El Mahyaoui R, Wattiez R, Azarkan M. A novel form of ficin from Ficus carica latex: Purification and characterization Phytochemistry. 2015 Sep;117:154-167.
6: Morellon-Sterling R, Siar EH, Braham SA, de Andrades D, Pedroche J, Millán MDC, Fernandez-Lafuente R. Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads J Biotechnol. 2021 Mar 10;329:128-142.
7: Chiang CL, Chang YM, Chang CT, Sung HY. Characterization of a chitosanase isolated from a commercial ficin preparation J Agric Food Chem. 2005 Sep 21;53(19):7579-85.
8: Azarkan M, Matagne A, Wattiez R, Bolle L, Vandenameele J, Baeyens-Volant D. Selective and reversible thiol-pegylation, an effective approach for purification and characterization of five fully active ficin (iso)forms from Ficus carica latex Phytochemistry. 2011 Oct;72(14-15):1718-31.
9: Malthouse JP, Brocklehurst K. Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2′-depyridyl disulphide as a reactivity probe Biochem J. 1976 Nov;159(2):221-34.
10: Stepanov VM, Rudenskaya GN. Proteinase affinity chromatography on bacitracin-Sepharose J Appl Biochem. 1983 Dec;5(6):420-8.

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