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5-hmC Glucosyltransferase

Original price was: $1,395.00.Current price is: $695.00.

Catalog Number: B2016581 (50 U)
5-hmC Glucosyltransferase is a high quality enzyme that catalyzes the transfer of a glucose molecule to the hydroxymethyl group of 5-hydroxymethylcytosine (5-hmC) in DNA. This enzymatic reaction is involved in the process of DNA demethylation, which plays a role in regulating gene expression and cellular differentiation. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

Live enquiry about this product via Text/SMS: 1-858-900-3210.

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Product Description

5-hmC Glucosyltransferase
Catalog number: B2016581
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 U
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: −20°C
Keywords: 5-hmC Glucosyltransferase
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Khare T, Pai S, Koncevicius K, Pal M, Kriukiene E, Liutkeviciute Z, Irimia M, Jia P, Ptak C, Xia M, Tice R, Tochigi M, Moréra S, Nazarians A, Belsham D, Wong AH, Blencowe BJ, Wang SC, Kapranov P, Kustra R, Labrie V, Klimasauskas S, Petronis A. 5-hmC in the brain is abundant in synaptic genes and shows differences at the exon-intron boundary Nat Struct Mol Biol. 2012 Oct;19(10):1037-43.
2: Terragni J, Bitinaite J, Zheng Y, Pradhan S. Biochemical characterization of recombinant β-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes Biochemistry. 2012 Feb 7;51(5):1009-19.
3: Liu M, Li CC, Luo X, Ma F, Zhang CY. 5-Hydroxymethylcytosine Glucosylation-Triggered Helicase-Dependent Amplification-Based Fluorescent Biosensor for Sensitive Detection of β-Glucosyltransferase with Zero Background Signal Anal Chem. 2020 Dec 15;92(24):16307-16313.
4: Yu M, Hon GC, Szulwach KE, Song CX, Jin P, Ren B, He C. Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine Nat Protoc. 2012 Dec;7(12):2159-70.
5: Robertson AB, Dahl JA, Ougland R, Klungland A. Pull-down of 5-hydroxymethylcytosine DNA using JBP1-coated magnetic beads Nat Protoc. 2012 Jan 26;7(2):340-50.
6: Jiang S, Shi H, Zhang Q, Wang ZY, Zhang Y, Zhang CY. Rolling circle transcription amplification-directed construction of tandem spinach-based fluorescent light-up biosensor for label-free sensing of β-glucosyltransferase activity Biosens Bioelectron. 2023 Oct 1;237:115513.
7: Yang Y , Yang G , Chen H , Zhang H , Feng JJ , Cai C . Electrochemical signal-amplified detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA using glucose modification coupled with restriction endonucleases Analyst. 2018 Apr 30;143(9):2051-2056.
8: Li Y, Song CX, He C, Jin P. Selective capture of 5-hydroxymethylcytosine from genomic DNA J Vis Exp. 2012 Oct 5;(68):4441.
9: Song CX, He C. Bioorthogonal labeling of 5-hydroxymethylcytosine in genomic DNA and diazirine-based DNA photo-cross-linking probes Acc Chem Res. 2011 Sep 20;44(9):709-17.
10: Chen S, Dou Y, Zhao Z, Li F, Su J, Fan C, Song S. High-Sensitivity and High-Efficiency Detection of DNA Hydroxymethylation in Genomic DNA by Multiplexing Electrochemical Biosensing Anal Chem. 2016 Apr 5;88(7):3476-80.

Products Related to 5-hmC Glucosyltransferase can be found at Enzymes

Additional Information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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