T7 RNA Polymerase (Highly Purified)

Product Description

T7 RNA Polymerase
Catalog number: B2010405
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5000 Units
Molecular Weight or Concentration: 99 kDa
Supplied as: Liquid Solution
Appearance: Clear Liquid
Applications: Highly purified and highly specific Bacteriophase T7 RNA Polymerase. This enzyme was expressed in E. coli and is provided with a 10X reaction buffer.
Storage: -20C
Keywords: T7 RNAP
Grade: Biotechnology grade. All solid components are highly pure (minimum 95%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Tabor S. Expression using the T7 RNA polymerase/promoter system. Curr Protoc
Mol Biol. 2001 May;Chapter 16:Unit16.2.

2: Borkotoky S, Murali A. The highly efficient T7 RNA polymerase: A wonder
macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt
A):49-56.

3: Passalacqua LFM, Dingilian AI, Lupták A. Single-pass transcription by T7 RNA
polymerase. RNA. 2020 Dec;26(12):2062-2071.

4: Kar S, Ellington AD. Construction of synthetic T7_RNA polymerase expression
systems. Methods. 2018 Jul 1;143:110-120.

5: Potapov V, Fu X, Dai N, Corrêa IR Jr, Tanner NA, Ong JL. Base modifications
affecting RNA polymerase and reverse transcriptase fidelity. Nucleic Acids Res.
2018 Jun 20;46(11):5753-5763.

6: Macdonald LE, Durbin RK, Dunn JJ, McAllister WT. Characterization of two
types of termination signal for bacteriophage T7_RNA polymerase. J Mol Biol.
1994 Apr 29;238(2):145-58.

7: Zhang X, Yao Z, Duan Y, Zhang X, Shi J, Xu Z. Investigation of specific
interactions between T7 promoter and T7_RNA polymerase by force spectroscopy
using atomic force microscope. Biochem J. 2018 Jan 11;475(1):319-328.

8: Gao W , Xu J , Lian G , Wang X , Gong X , Zhou D , Chang J . A novel
analytical principle using AP site-mediated T7_RNA polymerase transcription
regulation for sensing uracil-DNA glycosylase activity. Analyst. 2020 Jun
21;145(12):4321-4327.

9: Ji S, Thomforde J, Rogers C, Fu I, Broyde S, Tretyakova NY. Transcriptional
Bypass of DNA-Protein and DNA-Peptide Conjugates by T7_RNA Polymerase. ACS Chem
Biol. 2019 Dec 20;14(12):2564-2575.

10: Juhas M, Ajioka JW. T7 RNA_polymerase-driven inducible cell lysis for DNA
transfer from Escherichia coli to Bacillus subtilis. Microb Biotechnol. 2017
Nov;10(6):1797-1808.
11: Gholamalipour Y, Karunanayake Mudiyanselage A, Martin CT. 3′ end additions
by T7 RNA polymerase are RNA self-templated, distributive and diverse in
character-RNA-Seq analyses. Nucleic Acids Res. 2018 Oct 12;46(18):9253-9263.

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