4-Nitrophenyl Phosphate Di(tris) Salt


Catalog Number: B2012277 (100 mg)
4-Nitrophenyl Phosphate Di(tris) Salt is a high quality Substrate for Phosphatase. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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4-Nitrophenyl Phosphate Di(tris) Salt
Catalog number: B2012277
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 mg
Molecular Weight or Concentration: 461.4 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20C
Keywords: Phosphoric Acid 4-Nitrophenyl Ester Bis[tris(hydroxymethyl)aminomethane] Salt, 4-Nitrophenyl Phosphate Bis(tris) Salt
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Takayanagi T, Mine M, Mizuguchi H. Capillary Electrophoresis/Dynamic Frontal Analysis for the Enzyme Assay of 4-Nitrophenyl Phosphate with Alkaline Phosphatase Anal Sci. 2020 Jul 10;36(7):829-834.
2: Bowers GN Jr, McComb RB, Upretti A. 4-nitrophenyl phosphate–characterization of high-purity materials for measuring alkaline phosphatase activity in human serum Clin Chem. 1981 Jan;27(1):135-43.
3: Innocenti A, Supuran CT. Paraoxon, 4-nitrophenyl phosphate and acetate are substrates of α- but not of β-, γ- and ζ-carbonic anhydrases Bioorg Med Chem Lett. 2010 Nov 1;20(21):6208-12.
4: Li T, Zhong W, Jing C, Li X, Zhang T, Jiang C, Chen W. Enhanced Hydrolysis of p-Nitrophenyl Phosphate by Iron (Hydr)oxide Nanoparticles: Roles of Exposed Facets Environ Sci Technol. 2020 Jul 21;54(14):8658-8667.
5: Farrell HM Jr, Behe MJ, Enyeart JA. Binding of p-nitrophenyl phosphate and other aromatic compounds by beta-lactoglobulin J Dairy Sci. 1987 Feb;70(2):252-8.
6: Qi W, Fu Y, Zhao M, He H, Tian X, Hu L, Zhang Y. Electrochemiluminescence resonance energy transfer immunoassay for alkaline phosphatase using p-nitrophenyl phosphate as substrate Anal Chim Acta. 2020 Feb 8;1097:71-77.
7: Lacapère JJ, Garin J. Interaction of 4-azido-2-nitrophenyl phosphate, a pseudosubstrate, with the sarcoplasmic reticulum Ca-ATPase Biochemistry. 1994 Mar 8;33(9):2586-93.
8: Montalibet J, Skorey KI, Kennedy BP. Protein tyrosine phosphatase: enzymatic assays Methods. 2005 Jan;35(1):2-8.
9: Beaugé L, Berberián G, Campos M. Potassium-p-nitrophenyl phosphate interactions with (Na+ + K+)-ATPase. Their relevance to phosphatase activity Biochim Biophys Acta. 1984 Jun 13;773(1):157-64.
10: Pallen CJ, Wang JH. Calmodulin-stimulated dephosphorylation of p-nitrophenyl phosphate and free phosphotyrosine by calcineurin J Biol Chem. 1983 Jul 25;258(14):8550-3.

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