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A new interesting article has been published in Diagnostics (Basel). 2019 Jul 11;9(3). pii: E74. doi: 10.3390/diagnostics9030074. and titled:

Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor.

Authors of this article are:

Yrad FM, Castañares JM, Alocilja EC.

A summary of the article is shown below:

Dengue is a rapidly spreading mosquito-borne viral disease. Early diagnosis is important for clinical screening, medical management, and disease surveillance. The objective of this study was to develop a colorimetric lateral flow biosensor (LFB) for the visual detection of dengue-1 RNA using dextrin-capped gold nanoparticle (AuNP) as label. The detection was based on nucleic acid sandwich-type hybridization among AuNP-labeled DNA reporter probe, dengue-1 target RNA, and dengue-1 specific DNA capture probe immobilized on the nitrocellulose membrane. Positive test generated a red test line on the LFB strip, which enabled visual detection. The optimized biosensor has a cut-off value of 0.01 µM using synthetic dengue-1 target. Proof-of-concept application of the biosensor detected dengue-1 virus in pooled human sera with a cut-off value of 1.2 × 104 pfu/mL. The extracted viral RNA, when coupled with nucleic acid sequence-based amplification (NASBA), was detected on the LFB in 20 min. This study first demonstrates the applicability of dextrin-capped AuNP as label for lateral flow assay. The biosensor being developed provides a promising diagnostic platform for early detection of dengue infection in high-risk resource-limited areas.

Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/31336721 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: NASBA;dengue-1;dextrin-capped AuNP;lateral flow biosensor;sandwich-type hybridization.

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