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ELISA Microplate Production Kit

$495.00

Catalog #: K2010010
Kit containing all the required buffers to produce ELISA coated microplates: ELISA Coating Buffer, ELISA Washing Buffer and ELISA Blocking Buffer as well as microplate sealing films. Provided buffers need to be diluted with water before use. All kit components have been extensively validated for use in ELISA applications by coating various proteins, protein-conjugates andantibodies. This kit can be used in the production of ELISA coated microplates.

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SKU: K2010010 Categories: , ,

Description

ELISA Microplate Production Kit

Product Data Sheet Download PDF
MSDS Download PDF
Catalog number: K2010010
Lot number: Batch Dependent
Expiration date: Batch Dependent
Content: • ELISA Coating Buffer 5X (25 mL). Store at 2-8 °C.
• ELISA Washing Buffer 20X (50 mL). Store at room temperature.
• ELISA Blocking Buffer 10X concentrate (25 mL). Store at 2-8 °C.
• Microplates (10).
• Microplate Sealing Film (20).
Appearance: Clear to slightly white Solution
Application: ELISA
Shelf-Life 3 years
Storage: Room temperature and 2-8 °C.
Keywords: ELISA microplate coating, ELISA_microplate_production, ELISA kit

 

ELISA Coating/Washing/Blocking/Protocol

A detailed ELISA microplate production protocol with each kit.

References

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  • 2: Enzyme-linkedimmunosorbentassay (ELISA). Reen DJ. Methods Mol Biol. 1994;32:461-6.
  • 3: Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection. Arya SK, Estrela P. Sensors (Basel). 2018 Jun 22;18(7):2010.
  • 4: The application of immunoassay techniques, including enzyme-linkedimmunosorbentassay (ELISA), to snake venom research. Theakston RD. Toxicon. 1983;21(3):341-52.
  • 5: Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis. Brasil PE, Castro R, Castro Ld. Mem Inst Oswaldo Cruz. 2016 Jan;111(1):1-19.
  • 6: Lequin RM. Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay
    (ELISA). Clin Chem. 2005 Dec;51(12):2415-8.
  • 7: Aydin S. A short history, principles, and types of ELISA, and our laboratory
    experience with peptide/protein analyses using ELISA. Peptides. 2015
    Oct;72:4-15.
  • 8: Eble JA. Titration ELISA as a Method to Determine the Dissociation Constant
    of Receptor Ligand Interaction. J Vis Exp. 2018 Feb 15;(132):57334.
  • 9: Pang B, Zhao C, Li L, Song X, Xu K, Wang J, Liu Y, Fu K, Bao H, Song D, Meng
    X, Qu X, Zhang Z, Li J. Development of a low-cost paper-based ELISA method for
    rapid Escherichia coli O157:H7 detection. Anal Biochem. 2018 Feb 1;542:58-62.
  • 10: Major M, Law M. Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA.
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