ELISA Microplate Production Kit


Catalog #: K2010010
Kit containing all the required buffers to produce ELISA coated microplates: ELISA Coating Buffer, ELISA Washing Buffer and ELISA Blocking Buffer as well as microplate sealing films. Provided buffers need to be diluted with water before use. All kit components have been extensively validated for use in ELISA applications by coating various proteins, protein-conjugates and
antibodies. This kit can be used in the production of ELISA coated microplates.

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SKU: K2010010 Categories: , ,


ELISA Microplate Production Kit

Product Data Sheet Download PDF
MSDS Download PDF
Catalog number: K2010010
Lot number: Batch Dependent
Expiration date: Batch Dependent
Content: • ELISA Coating Buffer 5X (100 mL). Store at 2-8 °C.
• ELISA Washing Buffer 20X (50 mL). Store at room temperature.
• ELISA Blocking Buffer 10X concentrate (100 mL). Store at 2-8 °C.
• Microplate Sealing Film (50). Store at room temperature.
Appearance: Clear to slightly white Solution
Application: ELISA
Shelf-Life 3 years
Storage: Room temperature and 2-8 °C.
Keywords: ELISA microplate coating, ELISA microplate production, ELISA kit

ELISA Coating/Washing/Blocking/Protocol

A typical ELISA microplate production protocol is shown below:

  • Prepare the needed volume of ELISA Coating Buffer 1X by diluting the provided 5X concentrate using di-water.
  • Spike the protein/antibody to be coated inti the ELISA Coating Buffer 1X to the required concentration. Required concentration varies by application but is typically around 1 mg/mL (it can be, though, as low as 0.05 mg/mL and as high a 10 mg/mL). Mix until homogeneous.
  • Add 100 mL of the coating solution per microplate well either manually (using a multi-channel pipette) or using an automated liquid-handler.
  • Cover microplate with a sealing film (Catalog #CS20003).
  • Incubate at room temperature for 3 hours or at 2-8 °C
  • Remove the ELISA Coating Buffer (by sharply inverting the microplate into a container or using an automated liquid-handler).
  • Dispense 200 mL of ELISA Wash Buffer (Catalog #B2010009) into each microplate well. Remove ELISA Wash Buffer immediately. Remove any residual droplets by blotting plate on a piece of dry absorbent paper.
  • Dispense 250 mL of ELISA Blocking Buffer (Catalog #B2010010) to each microplate well. Cover microplate with a sealing film (Catalog #CS20003).
  • Incubate at room temperature for 2-3 hours.
  • Remove ELISA Blocking Buffer and any residual droplets by blotting plate on paper. Blocked microplates can be used directly for ELISA testing. If coated microplates are to be stored for the long term, they need to be dried and kept in sealed-bags (see steps 11-12).
  • Dry unsealed microplates in a 37°C incubator for 24-48 hours.
  • Place dry microplate in a foil sealable foil bag (Catalog #CS20001), add two Water Absorbent Packs (Catalog #CS20002). Seal the bag using a Teflon Heat Sealer of similar device.
  • Dried and sealed microplates are stable in original foil bags for at least 12 months at 2-8 °C.


  • Enzyme immunoassay and enzyme-linked immunosorbent assay. Gan SD, Patel KR. J Invest Dermatol. 2013 Sep;133(9):e12.
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  • Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection. Arya SK, Estrela P. Sensors (Basel). 2018 Jun 22;18(7):2010.
  • The application of immunoassay techniques, including enzyme-linkedimmunosorbentassay (ELISA), to snake venom research. Theakston RD. Toxicon. 1983;21(3):341-52.
  • Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis. Brasil PE, Castro R, Castro Ld. Mem Inst Oswaldo Cruz. 2016 Jan;111(1):1-19.

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