Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.

A new interesting article has been published in Diagn Microbiol Infect Dis. 2019 Jul;94(3):236-242. doi: 10.1016/j.diagmicrobio.2019.01.007. Epub 2019 Jan 16. and titled:

Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.

Authors of this article are:

Kenmoe S, Tcharnenwa C, Monamele GC, Kengne CN, Ripa MN, Whitaker B, Alroy KA, Balajee SA, Njouom R.

A summary of the article is shown below:

INTRODUCTION: This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test.METHODS: A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses.RESULTS: The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD® Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51).CONCLUSION: Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.Copyright © 2019 Elsevier Inc. All rights reserved.

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This article is a good source of information and a good way to become familiar with topics such as: Cameroon;Molecular diagnostic;Respiratory viruses.

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