E. coli PYKF Enzyme

Product Description

E. coli PYKF Enzyme
Catalog number: B2015143
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 ug
Molecular Weight or Concentration: 53.3 kDa (494 aa)
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -20 °C
Keywords: Pyruvate Kinase I, PK-1, PykF
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Dong H, Zhang J, Zhang H, Han Y, Lu C, Chen C, Tan X, Wang S, Bai X, Zhai G, Tian S, Zhang T, Cheng Z, Li E, Xu L, Zhang K. YiaC and CobB regulate lysine lactylation in Escherichia coli Nat Commun. 2022 Nov 4;13(1):6628.
2: Krylov AA, Airich LG, Kiseleva EM, Minaeva NI, Biryukova IV, Mashko SV. Conditional silencing of the Escherichia coli pykF gene results from artificial convergent transcription protected from Rho-dependent termination J Mol Microbiol Biotechnol. 2010;18(1):1-13.
3: Siddiquee KA, Arauzo-Bravo MJ, Shimizu K. Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli FEMS Microbiol Lett. 2004 Jun 1;235(1):25-33.
4: Bledig SA, Ramseier TM, Saier MH Jr. Frur mediates catabolite activation of pyruvate kinase (pykF) gene expression in Escherichia coli J Bacteriol. 1996 Jan;178(1):280-3.
5: Fang Z, Lai F, Cao K, Zhang Z, Cao L, Liu S, Duan Y, Yin X, Ge R, He QY, Sun X. Potential Role of Lysine Acetylation in Antibiotic Resistance of Escherichia coli mSystems. 2022 Dec 20;7(6):e0064922.
6: He S, Qi J, Yu S, Yin Y, Tan L, Bao S, Qiu X, Wang X, Fei R, Ding C. Expression and immunological characteristics of the surface-localized pyruvate kinase in Mycoplasma gallisepticum Microb Pathog. 2015 Dec;89:161-8.
7: Hofmann J, Heider C, Li W, Krausze J, Roessle M, Wilharm G. Recombinant production of Yersinia enterocolitica pyruvate kinase isoenzymes PykA and PykF Protein Expr Purif. 2013 Apr;88(2):243-7.
8: Al Zaid Siddiquee K, Arauzo-Bravo MJ, Shimizu K. Metabolic flux analysis of pykF gene knockout Escherichia coli based on 13C-labeling experiments together with measurements of enzyme activities and intracellular metabolite concentrations Appl Microbiol Biotechnol. 2004 Jan;63(4):407-17.
9: Zhao L, Lu Y, Yang J, Fang Y, Zhu L, Ding Z, Wang C, Ma W, Hu X, Wang X. Expression regulation of multiple key genes to improve L-threonine in Escherichia coli Microb Cell Fact. 2020 Feb 24;19(1):46.
10: Rodriguez A, Martínez JA, Báez-Viveros JL, Flores N, Hernández-Chávez G, Ramírez OT, Gosset G, Bolivar F. Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF Microb Cell Fact. 2013 Sep 30;12:86.

Products Related to E. coli PYKF Enzyme can be found at Enzymes