Diisopropyl-fluorophosphatase Enzyme

Product Description

Diisopropyl-fluorophosphatase Enzyme
Catalog number: B2016587
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: N/A
Supplied as: Powder
Applications: a molecular tool for various biochemical applications
Storage: −20°C
Keywords: Diisopropyl-fluorophosphatase
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Thakur M, Dean SN, Moore M, Spangler JR, Johnson BJ, Medintz IL, Walper SA. Packaging of Diisopropyl Fluorophosphatase (DFPase) in Bacterial Outer Membrane Vesicles Protects Its Activity at Extreme Temperature ACS Biomater Sci Eng. 2022 Feb 14;8(2):493-501.
2: Chen JC, Tonelli M, Anderson P, Michalczyk R, Blum MM, Williams RF. Backbone and side chain chemical shift assignment of diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris, an organophosphorus-degrading enzyme Biomol NMR Assign. 2023 Jun;17(1):55-60.
3: Zlobin A, Diankin I, Pushkarev S, Golovin A. Probing the Suitability of Different Ca(2+) Parameters for Long Simulations of Diisopropyl Fluorophosphatase Molecules. 2021 Sep 26;26(19):5839.
4: Melzer M, Heidenreich A, Dorandeu F, Gäb J, Kehe K, Thiermann H, Letzel T, Blum MM. In vitro and in vivo efficacy of PEGylated diisopropyl fluorophosphatase (DFPase) Drug Test Anal. 2012 Mar-Apr;4(3-4):262-70.
5: Mukherjee S, Gupta RD. Organophosphorus Nerve Agents: Types, Toxicity, and Treatments J Toxicol. 2020 Sep 22;2020:3007984.
6: Zhang H, Yang L, Ma YY, Zhu C, Lin S, Liao RZ. Theoretical Studies on Catalysis Mechanisms of Serum Paraoxonase 1 and Phosphotriesterase Diisopropyl Fluorophosphatase Suggest the Alteration of Substrate Preference from Paraoxonase to DFP Molecules. 2018 Jul 7;23(7):1660.
7: Blum MM, Chen JC. Structural characterization of the catalytic calcium-binding site in diisopropyl fluorophosphatase (DFPase)–comparison with related beta-propeller enzymes Chem Biol Interact. 2010 Sep 6;187(1-3):373-9.
8: Chen JC, Mustyakimov M, Schoenborn BP, Langan P, Blum MM. Neutron structure and mechanistic studies of diisopropyl fluorophosphatase (DFPase) Acta Crystallogr D Biol Crystallogr. 2010 Nov;66(Pt 11):1131-8.
9: Blum MM, Löhr F, Richardt A, Rüterjans H, Chen JC. Binding of a designed substrate analogue to diisopropyl fluorophosphatase: implications for the phosphotriesterase mechanism J Am Chem Soc. 2006 Oct 4;128(39):12750-7.
10: Gäb J, Melzer M, Kehe K, Wellert S, Hellweg T, Blum MM. Monitoring the hydrolysis of toxic organophosphonate nerve agents in aqueous buffer and in bicontinuous microemulsions by use of diisopropyl fluorophosphatase (DFPase) with (1)H- (31)P HSQC NMR spectroscopy Anal Bioanal Chem. 2010 Feb;396(3):1213-21.

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