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A new interesting article has been published in Clin Chem. 2019 Jul 1. pii: clinchem.2019.308122. doi: 10.1373/clinchem.2019.308122. and titled:

Topologic Analysis of Plasma Mitochondrial DNA Reveals the Coexistence of Both Linear and Circular Molecules.

Authors of this article are:

Ma ML, Zhang H, Jiang P, Sin STK, Lam WKJ, Cheng SH, Lee WS, Gai W, Tse OYO, Peng W, Wong J, Raghupathy R, Wong RSM, Sahota D, Leung TY, Chan KCA, Chiu RWK, Lo YMD.

A summary of the article is shown below:

BACKGROUND: Cellular mitochondrial DNA (mtDNA) is organized as a circular, covalently closed and double-stranded DNA. Studies have demonstrated the presence of short mtDNA fragments in plasma. It is not known whether circular mtDNA might concurrently exist with linear mtDNA in plasma.METHODS: We elucidated the topology of plasma mtDNA using restriction enzyme BfaI cleavage signatures on mtDNA fragment ends to differentiate linear and circular mtDNA. mtDNA fragments with both ends carrying BfaI cleavage signatures were defined as circular-derived mtDNA, whereas those with no cleavage signature or with 1 cleavage signature were defined as linear-derived mtDNA. An independent assay using exonuclease V to remove linear DNA followed by restriction enzyme MspI digestion was used for confirming the conclusions based on BfaI cleavage analysis. We analyzed the presence of BfaI cleavage signatures on plasma DNA ends in nonhematopoietically and hematopoietically derived DNA molecules by sequencing plasma DNA of patients with liver transplantation and bone marrow transplantation.RESULTS: Both linear and circular mtDNA coexisted in plasma. In patients with liver transplantation, donor-derived (i.e., liver) mtDNA molecules were mainly linear (median fraction, 91%; range, 75%-97%), whereas recipient-derived (i.e., hematopoietic) mtDNA molecules were mainly circular (median fraction, 88%; range, 77%-93%). The proportion of linear mtDNA was well correlated with liver DNA contribution in the plasma DNA pool (r = 0.83; P value = 0.0008). Consistent data were obtained from a bone marrow transplantation recipient in whom the donor-derived (i.e., hematopoietic) mtDNA molecules were predominantly circular.CONCLUSIONS: Linear and circular mtDNA molecules coexist in plasma and may have different tissue origins.© 2019 American Association for Clinical Chemistry.

Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/31263037 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: n/a.

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