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A new interesting article has been published in NMR Biomed. 2019 Jul 19:e4125. doi: 10.1002/nbm.4125. and titled:

The pH sensitivity of APT-CEST using phosphorus spectroscopy as a reference method.

Authors of this article are:

Schüre JR, Shrestha M, Breuer S, Deichmann R, Hattingen E, Wagner M, Pilatus U.

A summary of the article is shown below:

The pH value is a potential physiological marker for clinical diagnosis as it is altered in pathologies such as tumors. While intracellular pH can be measured noninvasively via phosphorus spectroscopy (31 P MRSI), Amide Proton Transfer-Chemical Exchange Saturation Transfer (APT-CEST) MRI has been suggested as an alternative method for pH quantification. To assess the suitability of APT-CEST contrast for pH quantification, two approaches (magnetization transfer ratio asymmetry [MTRasym ] and Lorentzian difference analysis [LDA]) for analyzing the Z-spectrum have been correlated with pH values obtained by 31 P MRSI. Fourteen patients with glioblastoma and 12 healthy controls were included. In contrast to MTRasym , the LDA is modeling the direct water saturation and the semi-solid magnetization transfer, allowing a separate evaluation of the aliphatic nuclear Overhauser effect and the APT-CEST. The results of our study show that the pH values obtained by 31 P MRSI correspond well with both methods describing the APT-CEST contrast. Two-sample t-test showed significant differences in MTRasym , LDA and pH obtained by 31 P MRSI for regions of interest in glioblastoma, contralateral control areas and normal appearing white matter (P < 0.001). A slightly improved correlation between the amide signal and pH was found after performing LDA (r = 0.78) compared with MTRasym (r = 0.70). While both methods can be used to monitor pH changes, the LDA approach appears to be better suited.© 2019 John Wiley & Sons, Ltd.
Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/31322308 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: Cellular and molecular cancer imaging;cellular and molecular imaging;chemical exchange saturation transfer;endogenous contrast methods;head and neck cancer;magnetization transfer;phosphorus magnetic resonance spectroscopy and spectroscopic imaging.

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