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A new interesting article has been published in ACS Appl Mater Interfaces. 2019 Aug 7;11(31):28469-28477. doi: 10.1021/acsami.9b08125. Epub 2019 Jul 26. and titled:

Liposome-Enhanced Polymerization-Based Signal Amplification for Highly Sensitive Naked-Eye Biodetection in Paper-Based Sensors.

Authors of this article are:

Kim S, Sikes HD.

A summary of the article is shown below:

Polymerization-based signal amplification (PBA) is a material-based approach to improving the sensitivity of paper-based diagnostic tests. Eosin Y is used as an assay label to photo-initiate free-radical polymerization to produce colored hydrogels in the presence of target analytes captured by bioactive paper. PBA achieves high-contrast and time-independent signals, but its nanomolar detection limit makes it impractical for early diagnosis of many diseases. In this work, we demonstrated efficient localization of large quantities of eosin Y per captured target analyte by incorporating eosin Y-loaded liposomes into PBA. This new “materials approach” allowed 30-fold signal enhancement compared to conventional PBA. To further improve the detection limit of liposome-enhanced PBA, we used a continuous flow-through assay format with 100 μL of analyte solution, achieving sub-nanomolar detection limits with high-contrast signals that were easily discernible to the unaided eye.

Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/31291078 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: colorimetric detection;dye-encapsulating liposome;paper-based diagnostic test;photo-initiated polymerization;signal amplification.

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