Luciferase from Photinus pyralis (firefly)

Product Description

Luciferase from Photinus pyralis (firefly)
Catalog number: B2016650
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 500 ug
Molecular Weight or Concentration: 62 kDa
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: −20°C
Keywords: Luciferase firefly
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Smale ST. Luciferase assay Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot5421.
2: de Wet JR, Wood KV, DeLuca M, Helinski DR, Subramani S. Firefly luciferase gene: structure and expression in mammalian cells Mol Cell Biol. 1987 Feb;7(2):725-37.
3: Branchini BR, Southworth TL, Fontaine DM, Davis AL, Behney CE, Murtiashaw MH. A Photinus pyralis and Luciola italica chimeric firefly luciferase produces enhanced bioluminescence Biochemistry. 2014 Oct 14;53(40):6287-9.
4: Hall MP, Unch J, Binkowski BF, Valley MP, Butler BL, Wood MG, Otto P, Zimmerman K, Vidugiris G, Machleidt T, Robers MB, Benink HA, Eggers CT, Slater MR, Meisenheimer PL, Klaubert DH, Fan F, Encell LP, Wood KV. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate ACS Chem Biol. 2012 Nov 16;7(11):1848-57.
5: Zhang H. HSV1-TK/GFP/Fluc 2008 Oct 21 [updated 2008 Dec 1]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013.
6: Smith AD, Trempe JP. Luminometric quantitation of photinus pyralis firefly luciferase and Escherichia coli beta-galactosidase in blood-contaminated organ lysates Anal Biochem. 2000 Nov 1;286(1):164-72.
7: Herbst R, Schäfer U, Seckler R. Equilibrium intermediates in the reversible unfolding of firefly (Photinus_pyralis) luciferase J Biol Chem. 1997 Mar 14;272(11):7099-105.
8: Herbst R, Gast K, Seckler R. Folding of firefly (Photinus_pyralis) luciferase: aggregation and reactivation of unfolding intermediates Biochemistry. 1998 May 5;37(18):6586-97.
9: Conti E, Lloyd LF, Akins J, Franks NP, Brick P. Crystallization and preliminary diffraction studies of firefly luciferase from Photinus_pyralis Acta Crystallogr D Biol Crystallogr. 1996 Jul 1;52(Pt 4):876-8.
10: Muthukumaran T, KrishnaMurthy NV, Sivaprasad N, Sudhaharan T. Isolation and characterization of luciferase from Indian firefly, Luciola praeusta Luminescence. 2014 Feb;29(1):20-8.

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