4-Nitrophenyl-β-D- galactosidase (p-Nitrophenyl-β-D-galactoside; PNP-β-D-Gal)

Product Description

4-Nitrophenyl-β-D- galactosidase (p-Nitrophenyl-β-D-galactoside; PNP-β-D-Gal)
Catalog number: B2015429
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 g
Molecular Weight or Concentration: 301.26 g/mol
Supplied as: powder
Applications: a molecular tool for various biochemical applications
Storage: -20°C
Keywords: p-Nitrophenyl-β-D-galactoside, PNP-β-D-Gal
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
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2: Ito K, Miyagawa K, Matsumoto M, Yabuno S, Kawakami N, Hamaguchi T, Iizuka M, Minamiura N. Evidence for the transglycosylation of complex type oligosaccharides of glycoproteins by endo-beta-N-acetylglucosaminidase HS Arch Biochem Biophys. 2006 Oct 1;454(1):89-99.
3: Kang HC, Lee SH. Characteristics of an alpha-galactosidase associated with grape flesh Phytochemistry. 2001 Sep;58(2):213-9.
4: Tomassi MH, Rozenfeld JH, Gonçalves LM, Marana SR. Characterization of the interdependency between residues that bind the substrate in a beta-glycosidase Braz J Med Biol Res. 2010 Jan;43(1):8-12.
5: Kis P, Potocká E, Mastihuba V, Mastihubová M. Efficient chemoenzymatic synthesis of 4-nitrophenyl β-d-apiofuranoside and its use in screening of β-d-apiofuranosidases Carbohydr Res. 2016 Jul 22;430:48-53.
6: Khan SH, Jain RK, Matta KL. Synthesis of some D-mannosyl-oligosaccharides containing the methyl and 4-nitrophenyl beta-D-mannopyranoside units Carbohydr Res. 1990 Oct 15;207(1):57-69.
7: Puchart V, Biely P. A simple enzymatic synthesis of 4-nitrophenyl beta-1,4-D-xylobioside, a chromogenic substrate for assay and differentiation of endoxylanases J Biotechnol. 2007 Feb 20;128(3):576-86.
8: Kanwar YS, Rosenzweig LJ, Jakubowski ML. Xylosylated-proteoglycan-induced Golgi alterations Proc Natl Acad Sci U S A. 1986 Sep;83(17):6499-503.
9: Biely P, Mastihubová M, la Grange DC, van Zyl WH, Prior BA. Enzyme-coupled assay of acetylxylan esterases on monoacetylated 4-nitrophenyl beta-D-xylopyranosides Anal Biochem. 2004 Sep 1;332(1):109-15.
10: Mastihubová M, Biely P. Lipase-catalysed preparation of acetates of 4-nitrophenyl beta-D-xylopyranoside and their use in kinetic studies of acetyl migration Carbohydr Res. 2004 May 17;339(7):1353-60.

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