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4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside

Original price was: $1,395.00.Current price is: $695.00.

Catalog Number: B2015018 (50 mg)
4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside is a high quality fluorogenic substrate for N-Acetyl-beta-D-glucosaminidase (NAGase), yielding a blue fluorescent solution. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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Product Description

4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside
Catalog number: B2015018
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mg
Molecular Weight or Concentration: 379.37 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20° C
Keywords: 4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Chow P, Weissmann B. 4-Methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside, a fluorogenic substrate for N-acetyl-alpha-D-glucosaminidase Carbohydr Res. 1981 Oct 1;96(1):87-93.
2: Zeng X, Sun Y, Uzawa H. Efficient enzymatic synthesis of 4-methylumbelliferyl N-acetyllactosaminide and 4-methylumbelliferyl sialyl N-acetyllactosaminides employing beta-D-galactosidase and sialyltransferases Biotechnol Lett. 2005 Oct;27(19):1461-5.
3: Marsh J, Fensom AH. 4-Methylumbelliferyl alpha-N-acetylglucosaminidase activity for diagnosis of Sanfilippo B disease Clin Genet. 1985 Mar;27(3):258-62.
4: Yuen CT, Kind PR, Price RG, Praill PF, Richardson AC. Colorimetric assay for N-acetyl-beta-D-glucosaminidase (NAG) in pathological urine using the omega-nitrostyryl substrate: the development of a kit and the comparison of manual procedure with the automated fluorimetric method Ann Clin Biochem. 1984 Jul;21 ( Pt 4):295-300.
5: el Moudni B, Rodier MH, Jacquemin JL. Purification and characterization of N-acetylglucosaminidase from Trypanosoma cruzi Exp Parasitol. 1996 Jul;83(2):167-73.
6: Shivakumar S, Karmali AN, Ruhimbana C. Partial purification, characterization, and kinetic studies of a low-molecular-weight, alkali-tolerant chitinase enzyme from Bacillus subtilis JN032305, A potential biocontrol strain Prep Biochem Biotechnol. 2014;44(6):617-32.
7: Hoell IA, Klemsdal SS, Vaaje-Kolstad G, Horn SJ, Eijsink VG. Overexpression and characterization of a novel chitinase from Trichoderma atroviride strain P1 Biochim Biophys Acta. 2005 May 15;1748(2):180-90.
8: Guetta E, Peleg L. Rapid detection of fetal Mendelian disorders: Tay-Sachs disease Methods Mol Biol. 2008;444:147-59.
9: O’Brien M, Colwell RR. A rapid test for chitinase activity that uses 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide Appl Environ Microbiol. 1987 Jul;53(7):1718-20.
10: Chang YM, Chen LC, Wang HY, Chiang CL, Chang CT, Chung YC. Characterization of an acidic chitinase from seeds of black soybean (Glycine max (L) Merr Tainan No. 3) PLoS One. 2014 Dec 1;9(12):e113596.

Products Related to 4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside can be found at Substrates

Additional Information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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