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A new interesting article has been published in J Virol Methods. 2019 Jul;269:43-48. doi: 10.1016/j.jviromet.2019.04.005. Epub 2019 Apr 5. and titled:

One-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil.

Authors of this article are:

Haach V, Gava D, Mauricio EC, Franco AC, Schaefer R.

A summary of the article is shown below:

Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based – one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV subtypes circulating in pigs in Brazil. The RT-qPCR assays presented 100% (95.7-100, CI 95%) of diagnostic sensitivity in the analysis of 85 IAVs, previously characterized by sequencing. The limits of detection ranged from 5.09 × 101 to 5.09 × 103 viral RNA copies/μL. For the analytical specificity, 73 pig samples collected during 2017 and 2018 were analyzed, resulting in the identification of the subtype in 74.0% (62.9-82.7, CI 95%) of samples. From these, 46.3% were H3N2, 33.3% were H1N1, 11.1% were H1N2 and 3.7% were HxN1. Mixed viral infections (3.7%) and reassortant viruses (1.9%) were also detected by the test. This multiplex RT-qPCR assay provides a fast and specific diagnostic tool for identification of different subtypes and lineages of IAV in pigs, contributing to the monitoring of influenza in swine.Copyright © 2019 Elsevier B.V. All rights reserved.

Check out the article’s website on Pubmed for more information:

[link-preview url=https://www.ncbi.nlm.nih.gov/pubmed/30959063 forceshot=true]

This article is a good source of information and a good way to become familiar with topics such as: Influenza A virus;Multiplex RT-qPCR;Rapid diagnosis;Subtyping;Swine.

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