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Equilibration Buffer for IPG Strips

Original price was: $1,395.00.Current price is: $695.00.

Catalog Number: B2016376 (50 mL)
Equilibration Buffer for IPG Strips is a high quality buffer supplied with a proprietary buffer necessary to support efficient reduction of the disulfide bridges and alkylation of the thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

Live enquiry about this product via Text/SMS: 1-858-900-3210.

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Product Description

Equilibration Buffer for IPG Strips
Catalog number: B2016376
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: N/A
Supplied as: Powder
Applications: a molecular tool for various biochemical applications
Storage: RT
Keywords: Equilibration Buffer for IPG Strips
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: McDonough J, Marbán E. Optimization of IPG strip equilibration for the basic membrane protein mABC1 Proteomics. 2005 Jul;5(11):2892-5.
2: Yan JX, Sanchez JC, Rouge V, Williams KL, Hochstrasser DF. Modified immobilized pH gradient gel strip equilibration procedure in SWISS-2DPAGE protocols Electrophoresis. 1999 Apr-May;20(4-5):723-6.
3: Stochaj WR, Berkelman T, Laird N. Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: IPG Strip Equilibration CSH Protoc. 2006 Oct 1;2006(5):pdb.prot4584.
4: Schüpbach J, Ammann RW, Freiburghaus AU. A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using isoelectric focusing on immobilized pH gradients in the first dimension Anal Biochem. 1991 Aug 1;196(2):337-43.
5: Ruan Y, Wan M. An optimized procedure for solubilization, reduction, and transfer of human breast cancer membrane-enriched fraction by 2-DE Electrophoresis. 2007 Sep;28(18):3333-40.
6: Wasinger VC, Bjellqvist B, Humphery-Smith I. Proteomic ‘contigs’ of Ochrobactrum anthropi, application of extensive pH gradients Electrophoresis. 1997 Aug;18(8):1373-83.
7: Li CM, William Hutchens T. Chromatofocusing Methods Mol Biol. 1992;11:237-48.
8: Kusnadi EP, Timpone C, Topisirovic I, Larsson O, Furic L. Regulation of gene expression via translational buffering Biochim Biophys Acta Mol Cell Res. 2022 Jan;1869(1):119140.
9: Schink B, Montag D, Keller A, Müller N. Hydrogen or formate: Alternative key players in methanogenic degradation Environ Microbiol Rep. 2017 Jun;9(3):189-202.
10: Gallagher FA, Kettunen MI, Brindle KM. Imaging pH with hyperpolarized 13C NMR Biomed. 2011 Oct;24(8):1006-15.

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Additional Information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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