Cas9 Protein (High Purity Enzyme)


Catalog Number: B2010495 (50 ug)
Cas9 Protein (High Purity) is a highly pure component of the (CRISPR)-RuvC (RNase H-like fold) cas system. This product has been used as a tool in functional genomics and genome editing. It has also been used in a wide array of other biochemical and immunological applications. Custom bulk amounts of this product are available upon request.

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Cas9 Protein (High Purity)
Catalog number: B2010495
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 ug
Molecular Weight or Concentration: 160 KD
Supplied as: Lyophilized powder
Applications: a tool in functional genomics and genome editing as well as other genetic and biochemical applications and uses.
Storage: −20°C
Keywords: SpCas9, SpyCas9, Cas9, cas5, csn1
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Mehta A, Merkel OM. Immunogenicity of Cas9 Protein. J Pharm Sci. 2020

2: Bondy-Denomy J. Protein Inhibitors of CRISPR-Cas9. ACS Chem Biol. 2018 Feb

3: Carmignotto GP, Azzoni AR. On the expression of recombinant Cas_9 protein in
E. coli BL21(DE3) and BL21(DE3) Rosetta strains. J Biotechnol. 2019 Dec

4: Laustsen A, Bak RO. Electroporation-Based CRISPR/Cas_9 Gene Editing Using Cas9
Protein and Chemically Modified sgRNAs. Methods Mol Biol. 2019;1961:127-134.

5: Xiao Q, Guo D, Chen S. Application of CRISPR/Cas_9-Based Gene Editing in
HIV-1/AIDS Therapy. Front Cell Infect Microbiol. 2019 Mar 22;9:69.

6: Khadempar S, Familghadakchi S, Motlagh RA, Farahani N, Dashtiahangar M,
Rezaei H, Gheibi Hayat SM. CRISPR-Cas_9 in genome editing: Its function and
medical applications. J Cell Physiol. 2019 May;234(5):5751-5761.

7: Suresh B, Ramakrishna S, Kim H. Cell-Penetrating Peptide-Mediated Delivery of
Cas_9 Protein and Guide RNA for Genome Editing. Methods Mol Biol.

8: Liu W, Yang C, Liu Y, Jiang G. CRISPR/Cas_9 System and its Research Progress
in Gene Therapy. Anticancer Agents Med Chem. 2019;19(16):1912-1919.

9: Li L, Hu S, Chen X. Non-viral delivery systems for CRISPR/Cas9-based genome
editing: Challenges and opportunities. Biomaterials. 2018 Jul;171:207-218.
10: Walton RT, Christie KA, Whittaker MN, Kleinstiver BP. Unconstrained genome
targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr

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