BCIP/NBT Alkaline Phosphatase Substrate


Catalog Number: B2010973 (150 mL)
BCIP/NBT Alkaline Phosphatase Substrate is a high quality two-component chromogenic substrate system for AP-based blotting. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other biochemical and immunological applications. Custom bulk amounts of this product are available upon request.

Live enquiry about this product via Text/SMS: 1-858-900-3210.

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BCIP/NBT Alkaline Phosphatase Substrate
Catalog number: B2010973
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 150 mL
Molecular Weight or Concentration: na
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: 5-bromo-4-chloro-3-indoyl-phosphate/nitroblue tetrazolium
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Jérôme V, Freitag R, Schüler D, Mickoleit F. SEAP activity measurement in reporter cell-based assays using BCIP / NBT as substrate Anal Biochem. 2019 Nov 15;585:113402.
2: Rodig SJ. Detecting Alkaline Phosphatase-Labeled Cells Cold Spring Harb Protoc. 2019 Apr 1;2019(4).
3: Dlouhy O, Kusnierova P, Kurasova I, Cisarikova M, Zeman D. Chemiluminescent detection of oligoclonal immunoglobulins after isoelectric focusing and affinity-mediated immunoblotting Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2018 Jun;162(2):107-115.
4: Bleckmann A, Dresselhaus T. Whole Mount RNA-FISH on Ovules and Developing Seeds Methods Mol Biol. 2017;1669:159-171.
5: Ryseff JK, Bohn AA. Detection of alkaline phosphatase in canine cells previously stained with Wright-Giemsa and its utility in differentiating osteosarcoma from other mesenchymal tumors Vet Clin Pathol. 2012 Sep;41(3):391-5.
6: West S, Schröder J, Kunz W. A multiple-staining procedure for the detection of different DNA fragments on a single blot Anal Biochem. 1990 Nov 1;190(2):254-8.
7: Panferov VG, Safenkova IV, Varitsev YA, Zherdev AV, Dzantiev BB. Enhancement of lateral flow immunoassay by alkaline phosphatase: a simple and highly sensitive test for potato virus X Mikrochim Acta. 2017 Dec 6;185(1):25.
8: Bronstein I, Voyta JC, Edwards B. A comparison of chemiluminescent and colorimetric substrates in a hepatitis B virus DNA hybridization assay Anal Biochem. 1989 Jul;180(1):95-8.
9: Lee SW, Lee KS, Ahn J, Lee JJ, Kim MG, Shin YB. Highly sensitive biosensing using arrays of plasmonic Au nanodisks realized by nanoimprint lithography ACS Nano. 2011 Feb 22;5(2):897-904.
10: Cacao EE, Nasrullah A, Sherlock T, Kemper S, Kourentzi K, Ruchhoeft P, Stein GE, Willson RC. High-resolution, high-throughput, positive-tone patterning of poly(ethylene glycol) by helium beam exposure through stencil masks PLoS One. 2013 May 24;8(5):e56835.

Products Related to BCIP/NBT Alkaline Phosphatase Substrate : Buffers & Solutions

Additional information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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4- Order by phone: 1-858-900-3210 (6 am – 8 pm PST).

5- Order by Text/SMS: 1-858-900-3210 (6 am – 8 pm PST).