X-Gal (Highly Pure)

Product Description

X-Gal (Highly Pure)
Catalog number: B2012758
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 mg
Molecular Weight or Concentration: 408.6 g/mol
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: BCIG, X-β-Gal, 5-bromo-4-chloro-3-indolyl-β-D-Galactopyranoside
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Green MR, Sambrook J. Screening Bacterial Colonies Using X-Gal and IPTG: α-Complementation Cold Spring Harb Protoc. 2019 Dec 2;2019(12).
2: Burn SF. Detection of β-galactosidase activity: X-gal staining Methods Mol Biol. 2012;886:241-50.
3: Itahana K, Campisi J, Dimri GP. Methods to detect biomarkers of cellular senescence: the senescence-associated beta-galactosidase assay Methods Mol Biol. 2007;371:21-31.
4: Sanchez-Ramos J, Song S, Dailey M, Cardozo-Pelaez F, Hazzi C, Stedeford T, Willing A, Freeman TB, Saporta S, Zigova T, Sanberg PR, Snyder EY. The X-gal caution in neural transplantation studies Cell Transplant. 2000 Sep-Oct;9(5):657-67.
5: Trimborn L, Hoecker U, Ponnu J. A Simple Quantitative Assay for Measuring β-Galactosidase Activity Using X_Gal in Yeast-Based Interaction Analyses Curr Protoc. 2022 May;2(5):e421.
6: Watanabe-Takano H, Fukumoto M, Fukuhara S, Mochizuki N. Protocol for whole-mount X-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC STAR Protoc. 2022 Jan 20;3(1):101127.
7: Kobayashi A. Imaging X-gal-Stained Mouse Embryos CSH Protoc. 2007 Apr 1;2007:pdb.prot4724.
8: Pati S, Jain S, Behera M, Acharya AP, Panda SK, Senapati S. X-gal staining of canine skin tissues: A technique with multiple possible applications J Nat Sci Biol Med. 2014 Jul;5(2):245-9.
9: Lozano-Gerona J, García-Otín ÁL. ImageJ-based semiautomatic method to analyze senescence in cell culture Anal Biochem. 2018 Feb 15;543:30-32.
10: Kim MY, Park RY, Bai YH, Chung YY, Kim CM, Kim SY, Rhee JH, Shin SH. X-gal inhibits the swarming of Vibrio species J Microbiol Methods. 2006 Sep;66(3):552-5.

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