Protein G Agarose

Description

Protein G Agarose
Catalog number: B2011105
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: molecular tool for various biochemical applications
Storage: 2-8 °C
Keywords: Protein G-agarose
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Grodzki AC, Berenstein E. Antibody purification: affinity chromatography – protein A and protein G Sepharose Methods Mol Biol. 2010;588:33-41.
2: Bonifacino JS, Dell’Angelica EC, Springer TA. Immunoprecipitation Curr Protoc Immunol. 2001 May;Chapter 8:Unit 8.3.
3: Zarrineh M, Mashhadi IS, Farhadpour M, Ghassempour A. Mechanism of antibodies purification by protein A Anal Biochem. 2020 Nov 15;609:113909.
4: Akerström B, Brodin T, Reis K, Björck L. Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies J Immunol. 1985 Oct;135(4):2589-92.
5: Wang Y, Zhang X, Han N, Wu Y, Wei D. Oriented covalent immobilization of recombinant protein A on the glutaraldehyde activated agarose support Int J Biol Macromol. 2018 Dec;120(Pt A):100-108.
6: Hirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP. Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation Anal Biochem. 2002 Sep 15;308(2):343-57.
7: Salehi N, Peng CA. Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography Biotechnol Prog. 2016 Jul 8;32(4):949-58.
8: Jungbauer A, Tauer C, Reiter M, Purtscher M, Wenisch E, Steindl F, Buchacher A, Katinger H. Comparison of protein A, protein G and copolymerized hydroxyapatite for the purification of human monoclonal antibodies J Chromatogr. 1989 Aug 4;476:257-68.
9: Bonifacino JS, Dell’Angelica EC, Springer TA. Immunoprecipitation Curr Protoc Mol Biol. 2001 May;Chapter 10:Unit 10.16.
10: Sun S, Tang Y, Fu Q, Liu X, Guo L, Zhao Y, Chang C. Monolithic cryogels made of agarose-chitosan composite and loaded with agarose beads for purification of immunoglobulin G Int J Biol Macromol. 2012 May 1;50(4):1002-7.

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