Fructosyl Amine-Oxygen Oxidoreductase


Catalog Number: P2010030 (100 U)
Fructosyl Amine-Oxygen Oxidoreductase is a highly pure enzyme (specific activity > 3U/mg) that catalyzes the oxidative deglycation of glycated low molecular weight amino acids to produce amino acids, glucosone and hydrogen peroxide (H2O2). This product has been used as a key component in glycated hemoglobin HbA1c assays. It has also been used in a wide array of other biochemical applications. Custom bulk amounts and bulk pricing for this product are available upon request.

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SKU: P2010030 Categories: ,


Fructosyl Amine-Oxygen Oxidoreductase
Catalog number: P2010030
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 U
Molecular Weight or Concentration: > 3 U/mg (~ 88 kDa). One unit will produce 1.0 μmole of hydrogen peroxide per minute at pH 8.0 at 37 °C
Supplied as: Lyophilized Powder
Applications: a component in glycated hemoglobin HbA1c assays
Storage: −20°C
Keywords: Fructosyl amine:oxygen oxidoreductase, fructosyl amino acid oxidase, amadoriase, EC 1.5.3, EC 1.5.3.X, fructosyl-a-l-amino acid:oxygen oxidoreductase, FAOD
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Ferri S, Kim S, Tsugawa W, Sode K. Review of fructosyl amino acid oxidase
engineering research: a glimpse into the future of hemoglobin A1c biosensing. J
Diabetes Sci Technol. 2009 May 1;3(3):585-92.

2: Sakaue R, Hiruma M, Kajiyama N, Koyama Y. Cloning and expression of
fructosyl-amino acid oxidase gene from Corynebacterium sp. 2-4-1 in Escherichia
coli. Biosci Biotechnol Biochem. 2002 Jun;66(6):1256-61.

3: Jeong HY, Song MH, Back JH, Han DM, Wu X, Monnier V, Jahng KY, Chae KS. The
veA gene is necessary for the inducible expression by fructosyl amines of the
Aspergillus nidulans faoA gene encoding fructosyl amino acid oxidase
(amadoriase, EC 1.5.3). Arch Microbiol. 2002 Nov;178(5):344-50.

4: Hirokawa K, Kajiyama N. Recombinant agrobacterium AgaE-like protein with
fructosyl amino acid oxidase activity. Biosci Biotechnol Biochem. 2002

5: Yoshida N, Takatsuka K, Katsuragi T, Tani Y. Occurrence of fructosyl-amino
acid oxidase-reactive compounds in fungal cells. Biosci Biotechnol Biochem. 2005

6: Fujiwara M, Sumitani J, Koga S, Yoshioka I, Kouzuma T, Imamura S, Kawaguchi
T, Arai M. Alteration of substrate specificity of fructosyl-amino acid oxidase
from Fusarium oxysporum. Appl Microbiol Biotechnol. 2007 Mar;74(4):813-9.

7: Yoshida N, Akazawa S, Katsuragi T, Tani Y. Characterization of two fructosyl-
amino acid oxidase homologs of Schizosaccharomyces pombe. J Biosci Bioeng.

8: Yoshida N, Sakai Y, Serata M, Tani Y, Kato N. Distribution and properties of
fructosyl amino acid oxidase in fungi. Appl Environ Microbiol. 1995

9: Sakaue R, Kajiyama N. Thermostabilization of bacterial fructosyl-amino acid
oxidase by directed evolution. Appl Environ Microbiol. 2003 Jan;69(1):139-45.
10: Yoshida N, Akazawa S, Karino T, Ishida H, Hata Y, Katsuragi T, Tani Y,
Takagi H. Functional analysis of genes encoding putative oxidoreductases in
Aspergillus oryzae, which are similar to fungal fructosyl-amino acid oxidase. J
Biosci Bioeng. 2007 Nov;104(5):424-7.

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