Tris Tricine SDS Buffer 10X Solution


Catalog Number: B2010112 (100 mL)
Tris-Tricine-SDS Buffer 10X is a high quality research product used as running buffer for polyacrylamide based separation of bronchoalveolar lavage fluids as well as low molecular weight proteins. Custom bulk orders of this product are available upon request.

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SKU: B2010112 Category: Tag:


Tris-Tricine-SDS Buffer 10X
Catalog number: B2010112
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 100 mL
pH: 8.2
Supplied as: 10X concentrate (dilute to 1X with di-water before use)
Appearance: Clear solution
Applications: running buffer for polyacrylamide based separation of bronchoalveolar lavage fluids as well as low molecular weight proteins.
Storage: Room temperature
Keywords: Tris-Tricine-SDS, TTS, Low molecular weight SDS-PAGE
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

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of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl
sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected
proteins. Anal Biochem. 1995 Jan 1;224(1):263-9.

2: Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining
of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006

3: Marshall T, Williams KM. Total protein determination in urine: elimination of
a differential response between the coomassie blue and pyrogallol red protein
dye-binding assays. Clin Chem. 2000 Mar;46(3):392-8.

4: Lim CW, Chisnall WN, Stokes YM, Pratt R, Crooke MJ. Effects of sodium
dodecylsulphate, dye concentration and paraprotein on coomassie blue dye-binding
assays for protein in urine. Clin Biochem. 1988 Oct;21(5):277-81.

5: Fujita T, Toda T, Ohashi M. Enzyme-linked immunodetection of proteins on
Coomassie blue-stained two-dimensional cellulose acetate membranes. Anal
Biochem. 1986 Nov 15;159(1):8-11.

6: Fountoulakis M, Juranville JF, Manneberg M. Comparison of the Coomassie
brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-
glycosylated and glycosylated proteins. J Biochem Biophys Methods. 1992

7: Spector T. Refinement of the coomassie blue method of protein quantitation. A
simple and linear spectrophotometric assay for less than or equal to 0.5 to 50
microgram of protein. Anal Biochem. 1978 May;86(1):142-6.

Products Related to Tris-Tricine-SDS Buffer 10X: Buffers and Solutions

Additional information

Weight 48 oz
Dimensions 8 × 8 × 8 in

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