Tris Tricine SDS Buffer 10X Solution

Description

Tris-Tricine-SDS Buffer 10X
Catalog number: B2010112
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 100 mL
pH: 8.2
Supplied as: 10X concentrate (dilute to 1X with di-water before use)
Appearance: Clear solution
Applications: running buffer for polyacrylamide based separation of bronchoalveolar lavage fluids as well as low molecular weight proteins.
Storage: Room temperature
Keywords: Tris-Tricine-SDS, TTS, Low molecular weight SDS-PAGE
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Fernandez-Patron C, Hardy E, Sosa A, Seoane J, Castellanos L. Double staining
of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl
sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected
proteins. Anal Biochem. 1995 Jan 1;224(1):263-9.

2: Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining
of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006
Feb;67(2):358-62.

3: Marshall T, Williams KM. Total protein determination in urine: elimination of
a differential response between the coomassie blue and pyrogallol red protein
dye-binding assays. Clin Chem. 2000 Mar;46(3):392-8.

4: Lim CW, Chisnall WN, Stokes YM, Pratt R, Crooke MJ. Effects of sodium
dodecylsulphate, dye concentration and paraprotein on coomassie blue dye-binding
assays for protein in urine. Clin Biochem. 1988 Oct;21(5):277-81.

5: Fujita T, Toda T, Ohashi M. Enzyme-linked immunodetection of proteins on
Coomassie blue-stained two-dimensional cellulose acetate membranes. Anal
Biochem. 1986 Nov 15;159(1):8-11.

6: Fountoulakis M, Juranville JF, Manneberg M. Comparison of the Coomassie
brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-
glycosylated and glycosylated proteins. J Biochem Biophys Methods. 1992
Jun;24(3-4):265-74.

7: Spector T. Refinement of the coomassie blue method of protein quantitation. A
simple and linear spectrophotometric assay for less than or equal to 0.5 to 50
microgram of protein. Anal Biochem. 1978 May;86(1):142-6.

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