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ELISA Wash Buffer (20X Solution)

Original price was: $795.00.Current price is: $395.00.

Catalog Number: B2010009 (50 mL)
ELISA Wash Buffer (20X Solution) is a concentrated liquid reagent specifically formulated to remove unbound and unnecessary components from microtiter plates during various stages of ELISA-based immunoassays. This optimized buffer helps reduce non-specific binding and background interference without disrupting intended antigen-antibody interactions, thereby improving the signal-to-noise ratio and assay reproducibility. Supplied as a 50 mL 20X concentrate, it must be diluted with deionized water to a 1X working solution before use and is compatible with manual or automated plate-washing systems.

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Product Description

4.9/5 - (12 votes)

ELISA Wash Buffer (20X)

Catalog number: B2010009
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume: 50 mL
Appearance: Clear Solution
Application: ELISA
Shelf-Life 3 years
Storage: Room temperature
Keywords: Elisa washing protocol, ELISA washing buffer, washing Elisa plates, washing buffer Elisa.

ELISA Coating/Washing/Blocking Protocol

Detailed protocol is provided.

References

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  • 4: The application of immunoassay techniques, including enzyme-linkedimmunosorbentassay (ELISA), to snake venom research. Theakston RD. Toxicon. 1983;21(3):341-52.
  • 5: Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis. Brasil PE, Castro R, Castro Ld. Mem Inst Oswaldo Cruz. 2016 Jan;111(1):1-19.
  • 6: Lequin RM. Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay
    (ELISA). Clin Chem. 2005 Dec;51(12):2415-8.
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    experience with peptide/protein analyses using ELISA. Peptides. 2015
    Oct;72:4-15.
  • 8: Eble JA. Titration ELISA as a Method to Determine the Dissociation Constant
    of Receptor Ligand Interaction. J Vis Exp. 2018 Feb 15;(132):57334.
  • 9: Pang B, Zhao C, Li L, Song X, Xu K, Wang J, Liu Y, Fu K, Bao H, Song D, Meng
    X, Qu X, Zhang Z, Li J. Development of a low-cost paper-based ELISA method for
    rapid Escherichia coli O157:H7 detection. Anal Biochem. 2018 Feb 1;542:58-62.
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Additional Information

Weight 2.6 oz
Dimensions 3.3 × 1.2 × 1.2 in
4.9/5 - (12 votes)

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