T4 DNA Polymerase

Product Description

T4 DNA Polymerase
Catalog number: B2016331
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 U
Molecular Weight or Concentration: 104 kDa
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -20 °C
Keywords: T4 DNA Polymerase
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Karam JD, Konigsberg WH. DNA_polymerase of the T4-related bacteriophages Prog Nucleic Acid Res Mol Biol. 2000;64:65-96.
2: Wang Y, Li B, Liu J, Zhou H. T4_DNA_polymerase-assisted upgrade of a nicking/polymerization amplification strategy for ultrasensitive electrochemical detection of Watermelon mosaic virus Anal Bioanal Chem. 2019 May;411(13):2915-2924.
3: Lo CY, Gao Y. DNA Helicase-Polymerase Coupling in Bacteriophage DNA Replication Viruses. 2021 Aug 31;13(9):1739.
4: Qi R, Otting G. Mutant T4 DNA polymerase for easy cloning and mutagenesis PLoS One. 2019 Jan 23;14(1):e0211065.
5: Li MZ, Elledge SJ. SLIC: a method for sequence- and ligation-independent cloning Methods Mol Biol. 2012;852:51-9.
6: Andrake M, Guild N, Hsu T, Gold L, Tuerk C, Karam J. DNA polymerase of bacteriophage T4 is an autogenous translational repressor Proc Natl Acad Sci U S A. 1988 Nov;85(21):7942-6.
7: Benkovic SJ, Spiering MM. Understanding DNA replication by the bacteriophage T4 replisome J Biol Chem. 2017 Nov 10;292(45):18434-18442.
8: Islam MN, Lee KW, Yim HS, Lee SH, Jung HC, Lee JH, Jeong JY. Optimizing T4_DNA_polymerase conditions enhances the efficiency of one-step sequence- and ligation-independent cloning Biotechniques. 2017 Sep 1;63(3):125-130.
9: Keohavong P, Kat AG, Cariello NF, Thilly WG. DNA amplification in vitro using T4_DNA_polymerase DNA. 1988 Jan-Feb;7(1):63-70.
10: Young MC, Reddy MK, von Hippel PH. Structure and function of the bacteriophage T4 DNA polymerase holoenzyme Biochemistry. 1992 Sep 22;31(37):8675-90.

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