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Beta-galactosidase Enzyme Donor

$795.00 $450.00

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Beta-galactosidase Enzyme Donor

$795.00 $450.00

Catalog #: P2010004 (1 mg)

Beta-galactosidase Enzyme Donor (also known as Alpha Peptide fragment) can complement the Beta-galactosidase Enzyme Acceptor (also known as Omega domain) to reconstitute an active Beta-galactosidase enzyme. This phenomenon, known as alpha complementation, can be exploited to develop assays by conjugating the Beta-galactosidase Alpha Peptide fragment to various analytes. The sequence of the enzyme donor has been specifically engineered to include a unique central cysteine that can be used for analyte conjugation.

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SKU: P2010004 Category: Tag:

Description

Beta-galactosidase Enzyme Donor (alpha peptide)

Product Data Sheet
Catalog # P2010004
Size 1.0 mg
Other Names Beta-galactosidase Alpha Peptide
Supplied as White lyophilized powder.
Molecular Weight 11 kDa (99 amino acids)
Purity >95% (SDS PAGE)
Storage -20°C. Avoid repeated freeze/thaw cycles.
Suggested buffer Beta-galactosidase Enzyme-Donor Stabilization Buffer (B2010001)
Keywords Beta-galactosidase Alpha Peptide, Enzyme Donor, alpha complementation, LacZ.
Related products Beta-galactosidase Enzyme Acceptor (Omega Domain), Beta-galactosidase Enzyme-Donor Stabilization Buffer, Beta-galactosidase Alpha Complementation Kit.

References

  • Kras, E. (2019). Beta-galactosidase: properties, structure and functions. New York: Nova Science Publishers.
  • Arndt, T. (2017). Cloned Enzyme-Donor Immunoassay. Lexikon Der Medizinischen Laboratoriumsdiagnostik, 1–2.
  • Jeon, S. I., Yang, X., and Andrade, J. D. (2004). Modeling of homogeneous cloned enzyme-donor immunoassay. Analytical Biochemistry, 333(1), 136–147.
  • Tachi, T., Kaji, N., Tokeshi, M., and Baba, Y. (2009). Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme-Donor. Analytical Sciences, 25(2), 149–151.
  • Khanna, P. L., and Worthy, T. E. (1993). CEDIA: A Recombinant-Based Homogeneous Enzyme Immunoassay.

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References

1- Matthews BW. The structure of E. coli beta-galactosidase. C R Biol. 2005 Jun;328(6):549-56.

2- Zeilstra-Ryalls JH, Somerville RL. Protein-protein interaction in the alpha-complementation system of beta-galactosidase. Curr Top Cell Regul. 1992;33:81-104.

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