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SDS Page Destaining Solution (100 mL)
$150.00
Catalog #: B2010020 (100 mL)
SDS Page Destaining Solution. Acetic acid/methanol solution suitable for destaining SDS PAGE gels and obtaining high contrast bands. Custom bulk orders are available upon request.
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Description
SDS Page Destaining Solution (100 mL)
| Catalog number: | B2010020B |
| Lot number: | Batch Dependent |
| Expiration date: | Batch Dependent |
| Volume: | 100 mL |
| Content | Methanol and acetic acid solution |
| Staining Time | 1 hour to overnight |
| Appearance: | Clear Solution |
| Application: | SDS PAGE gels destaining |
| Shelf-Life | Two years from date of manufacture |
| Storage: | Room temperature |
| Keywords: | Coomassie blue destsaining buffer, gel destaining buffer, SDS-PAGE destaining reagent, coomassie blue destaining, coomassie destain, SDS Page destaining Solution |
| Grade | Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. |
| References | 1: Fernandez-Patron C, Hardy E, Sosa A, Seoane J, Castellanos L. Double staining of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected proteins. Anal Biochem. 1995 Jan 1;224(1):263-9.2: Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006 Feb;67(2):358-62.3: Marshall T, Williams KM. Total protein determination in urine: elimination of a differential response between the coomassie blue and pyrogallol red protein dye-binding assays. Clin Chem. 2000 Mar;46(3):392-8.4: Lim CW, Chisnall WN, Stokes YM, Pratt R, Crooke MJ. Effects of sodium dodecylsulphate, dye concentration and paraprotein on coomassie blue dye-binding assays for protein in urine. Clin Biochem. 1988 Oct;21(5):277-81.5: Fujita T, Toda T, Ohashi M. Enzyme-linked immunodetection of proteins on Coomassie blue-stained two-dimensional cellulose acetate membranes. Anal Biochem. 1986 Nov 15;159(1):8-11.6: Fountoulakis M, Juranville JF, Manneberg M. Comparison of the Coomassie brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non- glycosylated and glycosylated proteins. J Biochem Biophys Methods. 1992 Jun;24(3-4):265-74.7: Spector T. Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein. Anal Biochem. 1978 May;86(1):142-6. |
| Related Products | Biochemical Buffers and Solutions |
Additional information
| Weight | 48 oz |
|---|---|
| Dimensions | 8 × 8 × 8 in |
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