TBE Buffer 10X (Tris Borate EDTA) (Biotechnology Grade)

$95.00

Catalog #: B2010012 (250 mL)
TBE Buffer (10X concentrate) used for polyacrylamide and agarose electrophoresis. A 1X solution is obtained by diluting 1 volume of the provided TBE 10X concentrated TBE to 9 volumes of deionized water.

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Description

TBE Buffer 10X (Tris Borate EDTA)

Catalog number: B2010012
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume: 250 mL
Content Tris-Borate+ EDTA
pH 8.3
Appearance: Clear Solution
Application: Polyacrylamide and Agarose electrophoresis (dilute to 1X before use)
Shelf-Life Two years from date of manufacture
Storage: Room temperature
Keywords: TBE solution, tris-borate EDTA buffer, TBE buffer, DNA electrophoresis buffer, TBE Buffer 10X Solution
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Strutz K, Stellwagen NC. Do DNA gel electrophoretic mobilities extrapolate to
the free-solution mobility of DNA at zero gel concentration? Electrophoresis.
1998 May;19(5):635-42. doi: 10.1002/elps.1150190504. PMID: 9629889.2: Stellwagen NC, Bossi A, Gelfi C, Righetti PG. DNA and buffers: are there any
noninteracting, neutral pH buffers? Anal Biochem. 2000 Dec 1;287(1):167-75. doi:
10.1006/abio.2000.4848. PMID: 11078596.3: Ungewickell E, Oestergaard L. Identification of the clathrin assembly protein
AP180 in crude calf brain extracts by two-dimensional sodium dodecyl sulfate-
polyacrylamide gel electrophoresis. Anal Biochem. 1989 Jun;179(2):352-6. doi:
10.1016/0003-2697(89)90143-7. PMID: 2774183.4: Hayes VM, Wu Y, Osinga J, Mulder IM, van der Vlies P, Elfferich P, Buys CH,
Hofstra RM. Improvements in gel composition and electrophoretic conditions for
broad-range mutation analysis by denaturing gradient gel electrophoresis.
Nucleic Acids Res. 1999 Oct 15;27(20):e29. doi: 10.1093/nar/27.20.e29. PMID:
10497279; PMCID: PMC148660.5: Stellwagen NC. Apparent pore size of polyacrylamide gels: comparison of gels
cast and run in Tris-acetate-EDTA and Tris-borate-EDTA buffers. Electrophoresis.
1998 Jul;19(10):1542-7. doi: 10.1002/elps.1150191004. PMID: 9719523.6: Stellwagen E, Stellwagen NC. Determining the electrophoretic mobility and
translational diffusion coefficients of DNA molecules in free solution.
Electrophoresis. 2002 Aug;23(16):2794-803. doi:
10.1002/1522-2683(200208)23:16<2794::AID-ELPS2794>3.0.CO;2-Y. PMID: 12210184.7: Ali RW, Martin L, Haffajee AD, Socransky SS. Detection of identical ribotypes
of Porphyromonas gingivalis in patients residing in the United States, Sudan,
Romania and Norway. Oral Microbiol Immunol. 1997 Apr;12(2):106-11. doi:
10.1111/j.1399-302x.1997.tb00625.x. PMID: 9227134.8: Common stock solutions, buffers, and media. Curr Protoc Cell Biol. 2001 May; Appendix 2:Appendix 2A.
Related Products Biochemical Buffers and Solutions

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