Nitroreductase from Escherichia coli

Description

Nitroreductase from Escherichia coli
Catalog number: B2012858
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 mg
Molecular Weight or Concentration: N/A
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: -20°C
Keywords: NTRA
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
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2: Christofferson A, Wilkie J. Mechanism of CB1954 reduction by Escherichia coli nitroreductase Biochem Soc Trans. 2009 Apr;37(Pt 2):413-8.
3: Le VVH, Davies IG, Moon CD, Wheeler D, Biggs PJ, Rakonjac J. Novel 5-Nitrofuran-Activating Reductase in Escherichia coli Antimicrob Agents Chemother. 2019 Oct 22;63(11):e00868-19.
4: Wan Y, Mills E, Leung RCY, Vieira A, Zhi X, Croucher NJ, Woodford N, Jauneikaite E, Ellington MJ, Sriskandan S. Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom Microb Genom. 2021 Dec;7(12):000702.
5: Parkinson GN, Skelly JV, Neidle S. Crystal structure of FMN-dependent nitroreductase from Escherichia coli B: a prodrug-activating enzyme J Med Chem. 2000 Oct 5;43(20):3624-31.
6: Anlezark GM, Melton RG, Sherwood RF, Coles B, Friedlos F, Knox RJ. The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)–I. Purification and properties of a nitroreductase enzyme from Escherichia coli–a potential enzyme for antibody-directed enzyme prodrug therapy (ADEPT) Biochem Pharmacol. 1992 Dec 15;44(12):2289-95.
7: Choi JW, Lee J, Nishi K, Kim YS, Jung CH, Kim JS. Crystal structure of a minimal nitroreductase_, ydjA, from Escherichia coli K12 with and without FMN cofactor J Mol Biol. 2008 Mar 14;377(1):258-67.
8: Emptage CD, Knox RJ, Danson MJ, Hough DW. Nitroreductase_from Bacillus licheniformis: a stable enzyme for prodrug activation Biochem Pharmacol. 2009 Jan 1;77(1):21-9.
9: Denny WA. Nitroreductase-based GDEPT Curr Pharm Des. 2002;8(15):1349-61.
10: Clarke TA, Mills PC, Poock SR, Butt JN, Cheesman MR, Cole JA, Hinton JC, Hemmings AM, Kemp G, Söderberg CA, Spiro S, Van Wonderen J, Richardson DJ. Escherichia coli cytochrome c nitrite reductase NrfA Methods Enzymol. 2008;437:63-77.

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