Monobromobimane (mBBr)


Catalog Number: B2011454 (5 mg)
Monobromobimane (mBBr) is a high quality thiol-reactive fluorescent dye. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

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Monobromobimane (mBBr)
Catalog number: B2011454
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5 mg
Molecular Weight or Concentration: 271.1 g/mol
Supplied as: Lyophilized
Applications: molecular tool for various biochemical applications
Storage: -20 °C
Keywords: mBBr
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

1: Srivastava A, Baidya M, Dwivedi-Agnihotri H, Shukla AK. Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors Methods Enzymol. 2020;633:271-280.
2: Ralat LA, Colman RF. Monobromobimane occupies a distinct xenobiotic substrate site in glutathione S-transferase pi Protein Sci. 2003 Nov;12(11):2575-87.
3: Ni T, Huang X, Pan S, Lu Z. Inhibition of the long non-coding RNA ZFAS1 attenuates ferroptosis by sponging miR-150-5p and activates CCND2 against diabetic cardiomyopathy J Cell Mol Med. 2021 Nov;25(21):9995-10007.
4: Kurpet K, Głowacki R, Chwatko G. Simultaneous Determination of Human Serum Albumin and Low-Molecular-Weight Thiols after Derivatization with Monobromobimane Molecules. 2021 Jun 1;26(11):3321.
5: Shen X, Kolluru GK, Yuan S, Kevil CG. Measurement of H2S in vivo and in vitro by the monobromobimane method Methods Enzymol. 2015;554:31-45.
6: Gonzalez-Andrade M, Rivera-Chavez J, Sosa-Peinado A, Figueroa M, Rodriguez-Sotres R, Mata R. Development of the fluorescent biosensor hCalmodulin (hCaM)L39C-monobromobimane(mBBr)/V91C-mBBr, a novel tool for discovering new calmodulin inhibitors and detecting calcium J Med Chem. 2011 Jun 9;54(11):3875-84.
7: Ditrói T, Nagy A, Martinelli D, Rosta A, Kožich V, Nagy P. Comprehensive analysis of how experimental parameters affect H(2)S measurements by the monobromobimane method Free Radic Biol Med. 2019 May 20;136:146-158.
8: Cabrillana ME, Uribe P, Villegas JV, Álvarez J, Sánchez R, Fornés MW. Thiol oxidation by nitrosative stress: Cellular localization in human spermatozoa Syst Biol Reprod Med. 2016 Oct;62(5):325-34.
9: Hashida SN, Kawai-Yamada M. Detection of Disulfides in Protein Extracts ofArabidopsis thaliana using Monobromobimane (mBB) Bio Protoc. 2019 Mar 5;9(5):e3183.
10: Weis M, Cotgreave IC, Moore GA, Norbeck K, Moldéus P. Accessibility of hepatocyte protein thiols to monobromobimane Biochim Biophys Acta. 1993 Mar 10;1176(1-2):13-9.

Products Related to Monobromobimane (mBBr) can be found at Fluorescence

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