Isopropyl-β-D-thiogalactoside IPTG (Lactose Based)

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Isopropyl-β-D-thiogalactoside IPTG (Lactose Based)
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Isopropyl-β-D-thiogalactoside IPTG (Lactose Based)

Original price was: $795.00.Current price is: $395.00.

Catalog Number: B2027245 (1 g)
Isopropyl-β-D-thiogalactoside (IPTG) is a molecular biology reagent used as a non-metabolizable inducer of the lac operon in Escherichia coli and other bacteria. By binding to and inactivating the lac repressor, IPTG triggers the transcription of genes under the control of lac promoters, making it essential for recombinant protein expression. This lactose-based reagent is supplied as a high-purity, stable compound widely utilized in biotechnology, biochemistry, and genetic engineering research. Custom bulk amounts of this product are available upon request.

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Product Description

5/5 - (1 vote)

Isopropyl-β-D-thiogalactoside IPTG (Lactose Based)
Catalog number: B2027245
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 g
Molecular Weight or Concentration: 238.31
Supplied as: Powder
Storage: NA
Keywords: Isopropyl-β-D-thiogalactopyranoside, Isopropyl-beta-D-thiogalactopyranoside, IPTG, Isopropyl thiogalactoside, Isopropyl β-D-1-thiogalactopyranoside

References:
1. Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science. 1996;271(5253):1247-1254.
2. Studier FW. Protein production by auto-induction in high-density shaking cultures. Protein Expression and Purification. 2005;41(1):207-234.
3. Faust G, Stand A, Weuster-Botz D. IPTG can replace lactose in auto-induction media to enhance protein expression in Escherichia coli. Journal of Biotechnology. 2015;210:38-40.
4. Hansen LH, Knudsen S, Sørensen SJ. The effect of the lacY gene on the induction of IPTG-inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens. Current Microbiology. 1998;36(6):341-347.
5. Donovan RS, Robinson CW, Glick BR. Review: optimizing inducer concentration and timing in E. coli fermentation processes. Biotechnology Progress. 1996;12(2):123-131.
6. Marbach A, Bettenbrock K. Lac operon induction in Escherichia coli: Systematic comparison of IPTG and TMG as inducers. Journal of Biotechnology. 2012;157(1):82-88.
7. Daber R, Stayrook S, Rosenberg A, Lewis M. Structural analysis of inducer binding in the Lac repressor. Journal of Molecular Biology. 2007;370(4):609-619.
8. Larentis AL, Nicolau JFA, de Lucca Freire M, et al. Evaluation of different carbon sources and induction strategies for recombinant protein production in Escherichia coli. Brazilian Journal of Chemical Engineering. 2014;31(2):333-346.
9. Ukkonen K, Veijola J, Vasala A, Neubauer P. High-yield production of recombinant proteins using auto-induction in shaking cultures. Microbial Cell Factories. 2013;12(1):58.
10. Briand L, Marison IW, von Stockar U. Lactose as a gratuitous inducer of the lac operon in Escherichia coli. Biotechnology and Bioengineering. 1999;62(5):548-555.
Isopropyl-β-D-thiogalactoside IPTG (lactose based)

Products Related to Isopropyl-β-D-thiogalactoside IPTG (Lactose Based) can be found at Carbohydrates

Additional Information

Weight 0.15 oz
Dimensions 2 × 0.5 × 0.5 in
5/5 - (1 vote)

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