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FnCas9 Protein (Highly Pure from Francisella Novicida)

$295.00

Catalog Number: B2010492 (50 ug)
FnCas9 Protein (Highly Pure) is a highly pure derivative of the type II CRISPR/Cas system from the bacterium Francisella novicida . This product has been used as a tool for plasmid and large genomic DNA fragment cloning. It has also been used in a wide array of other biochemical and immunological applications. Custom bulk amounts of this product are available upon request.

Live enquiry about this product via Text/SMS: 1-858-900-3210.

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SKU: B2010492 Categories: ,

Description

FnCas9 Protein (Highly Pure)
Catalog number: B2010492
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 ug
Molecular Weight or Concentration: 190 kDa
Supplied as: Lyophilized powder
Applications: a tool for plasmid and large genomic DNA fragment cloning
Storage: −20°C
Keywords: Francisella Novicida Cas9
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References:
1: Islam KU, Iqbal J. An Update on Molecular Diagnostics for COVID-19. Front
Cell Infect Microbiol. 2020 Nov 10;10:560616.

2: Green JC, Hu JS. Editing plants for virus resistance using CRISPR-Cas. Acta
Virol. 2017;61(2):138-142.

3: Hirano H, Gootenberg JS, Horii T, Abudayyeh OO, Kimura M, Hsu PD, Nakane T,
Ishitani R, Hatada I, Zhang F, Nishimasu H, Nureki O. Structure and Engineering
of Francisella novicida Cas9. Cell. 2016 Feb 25;164(5):950-61.

4: Kumar M, Gulati S, Ansari AH, Phutela R, Acharya S, Azhar M, Murthy J,
Kathpalia P, Kanakan A, Maurya R, Vasudevan JS, S A, Pandey R, Maiti S,
Chakraborty D. FnCas9-based CRISPR diagnostic for rapid and accurate detection
of major SARS-CoV-2 variants on a paper strip. Elife. 2021 Jun 9;10:e67130.

5: Azhar M, Phutela R, Kumar M, Ansari AH, Rauthan R, Gulati S, Sharma N, Sinha
D, Sharma S, Singh S, Acharya S, Sarkar S, Paul D, Kathpalia P, Aich M, Sehgal
P, Ranjan G, Bhoyar RC; Indian CoV2 Genomics & Genetic Epidemiology (IndiCovGEN)
Consortium, Singhal K, Lad H, Patra PK, Makharia G, Chandak GR, Pesala B,
Chakraborty D, Maiti S. Rapid and accurate nucleobase detection using FnCas9 and
its application in COVID-19 diagnosis. Biosens Bioelectron. 2021 Jul
1;183:113207.

6: Price AA, Sampson TR, Ratner HK, Grakoui A, Weiss DS. Cas9-mediated targeting
of viral RNA in eukaryotic cells. Proc Natl Acad Sci U S A. 2015 May
12;112(19):6164-9.

7: Mekler V, Kuznedelov K, Severinov K. Quantification of the affinities of
CRISPR-Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences. J
Biol Chem. 2020 May 8;295(19):6509-6517.

8: Acharya S, Mishra A, Paul D, Ansari AH, Azhar M, Kumar M, Rauthan R, Sharma
N, Aich M, Sinha D, Sharma S, Jain S, Ray A, Jain S, Ramalingam S, Maiti S,
Chakraborty D. Francisella novicida Cas9 interrogates genomic DNA with
very high specificity and can be used for mammalian genome editing. Proc Natl
Acad Sci U S A. 2019 Oct 15;116(42):20959-20968.

9: Chen F, Ding X, Feng Y, Seebeck T, Jiang Y, Davis GD. Targeted activation of
diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR
targeting. Nat Commun. 2017 Apr 7;8:14958.
10: Zhang T, Zheng Q, Yi X, An H, Zhao Y, Ma S, Zhou G. Establishing RNA virus
resistance in plants by harnessing CRISPR immune system. Plant Biotechnol J.
2018 Aug;16(8):1415-1423.

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