Secondary Antibody Dilution Buffer

$125.00

Catalog Number: B2010081 (250 mL)
Secondary Antibody Dilution Buffer is a versatile, very stable and reproducible diluent for secondary antibodies. It is compatible with antibodies conjugated to HRP and used in ELISA and similar immuno applications. Buffer is specially formulated to optimize the activity, the stability and the reproducibility of the HRP-generated signal. Custom bulk orders are available upon request.

In stock

SKU: B2010081 Categories: ,

Description

Secondary Antibody Dilution Buffer

Catalog number: B2010081
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 250 mL
pH 7.4
Supplied as Ready-to-use solution
Appearance clear solution
Applications General dilution buffer for secondary antibodies used in ELISA and similar immunoapplications. Buffer is compatible with HRP conjugates.
Shelf-Life 1 year from date of manufacture
Storage: Keep at 2-8°C
Keywords: Secondary antibody diluent, antibody conjugate diluent, HRP diluent
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Ge M, Li RC, Qu T, Gong W, Yu XL, Tu C. Construction of an HRP-streptavidin
bound antigen and its application in an ELISA for porcine circovirus 2
antibodies. AMB Express. 2017 Sep 18;7(1):177. doi: 10.1186/s13568-017-0473-3.
PMID: 28921455; PMCID: PMC5603472.

2: Poolperm P, Varinrak T, Kataoka Y, Tragoolpua K, Sawada T, Sthitmatee N.
Development and standardization of an in-house indirect ELISA for detection of
duck antibody to fowl cholera. J Microbiol Methods. 2017 Nov;142:10-14.

3: Panda R, Boyer M, Garber EAE. A multiplex competitive ELISA for the detection
and characterization of gluten in fermented-hydrolyzed foods. Anal Bioanal Chem.
2017 Dec;409(30):6959-6973.
4: Jadhav PD, Shim YY, Reaney MJ. Synthesis and Characterization of Site-
Selective Orbitide-BSA Conjugate to Produce Antibodies. Bioconjug Chem. 2016 Oct
19;27(10):2346-2358.

5: Kim WJ, Cho HY, Jeong B, Byun S, Huh J, Kim YJ. Synergistic Use of Gold
Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)”
for High Sensitivity and Fast Assays. Sensors (Basel). 2017 Dec 26;18(1):55.

6: Mitsui Y. Development of a simple and specific direct competitive ELISA for
the determination of artesunate using an anti-artesunate polyclonal antiserum.
Trop Med Health. 2016 Nov 21;44:37.

7: Al-Dujaili EA, Baghdadi HH, Howie F, Mason JI. Validation and application of
a highly specific and sensitive ELISA for the estimation of cortisone in saliva,
urine and in vitro cell-culture media by using a novel antibody. Steroids. 2012
May;77(6):703-9.

8: Al-Dujaili EA. Development and validation of a simple and direct ELISA method
for the determination of conjugated (glucuronide) and non-conjugated
testosterone excretion in urine. Clin Chim Acta. 2006 Feb;364(1-2):172-9.

Products Related to Secondary Antibody Dilution Buffer Biochemical Buffers and Solutions

Ordering Information

1- Order online using our website’s automated shopping cart and checkout system (available 24/7).

2- Order by email: info@moleculardepot.com (we accept electronic PO, available 24/7).

3- Order by Fax: 1-858-437-9884 (available 24/7).

4- Order by phone: 1-858-900-3210 (6 am – 8 pm PST).

5- Order by Text/SMS: 1-858-900-3210 (6 am – 8 pm PST).