Secondary Antibody Dilution Buffer
Original price was: $795.00.$395.00Current price is: $395.00.
Catalog Number: B2010081 (50 mL)
Secondary Antibody Dilution Buffer (50 mL) is a reagent designed to maintain the stability and functionality of secondary antibodies during immunostaining applications such as immunohistochemistry, immunocytochemistry, and Western blotting. The buffer is optimized to minimize non-specific binding and cross-reactivity, thereby reducing background noise and improving the signal-to-noise ratio in sensitive assays. This filtered, solution provides a physiological environment that preserves antibody binding activity for long-term storage at 2-8 ºC.
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Product Description
Secondary Antibody Dilution Buffer
| Catalog number: | B2010081 |
| Lot number: | Batch Dependent |
| Expiration date: | Batch Dependent |
| Volume/Weight | 50 mL |
| pH | 7.4 |
| Supplied as | Ready-to-use solution |
| Appearance | clear solution |
| Applications | General dilution buffer for secondary antibodies used in ELISA and similar immunoapplications. Buffer is compatible with HRP conjugates. |
| Shelf-Life | 1 year from date of manufacture |
| Storage: | Keep at 2-8°C |
| Keywords: | Secondary antibody diluent, antibody conjugate diluent, HRP diluent |
| Grade | Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. |
| References | 1: Ge M, Li RC, Qu T, Gong W, Yu XL, Tu C. Construction of an HRP-streptavidin bound antigen and its application in an ELISA for porcine circovirus 2 antibodies. AMB Express. 2017 Sep 18;7(1):177. doi: 10.1186/s13568-017-0473-3. PMID: 28921455; PMCID: PMC5603472.2: Poolperm P, Varinrak T, Kataoka Y, Tragoolpua K, Sawada T, Sthitmatee N. Development and standardization of an in-house indirect ELISA for detection of duck antibody to fowl cholera. J Microbiol Methods. 2017 Nov;142:10-14.3: Panda R, Boyer M, Garber EAE. A multiplex competitive ELISA for the detection and characterization of gluten in fermented-hydrolyzed foods. Anal Bioanal Chem. 2017 Dec;409(30):6959-6973. 4: Jadhav PD, Shim YY, Reaney MJ. Synthesis and Characterization of Site- Selective Orbitide-BSA Conjugate to Produce Antibodies. Bioconjug Chem. 2016 Oct 19;27(10):2346-2358. 5: Kim WJ, Cho HY, Jeong B, Byun S, Huh J, Kim YJ. Synergistic Use of Gold 6: Mitsui Y. Development of a simple and specific direct competitive ELISA for 7: Al-Dujaili EA, Baghdadi HH, Howie F, Mason JI. Validation and application of 8: Al-Dujaili EA. Development and validation of a simple and direct ELISA method |
| Products Related to Secondary Antibody Dilution Buffer | Biochemical Buffers and Solutions |
Additional Information
| Weight | 2.6 oz |
|---|---|
| Dimensions | 3.3 × 1.2 × 1.2 in |
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