CPRG (Chlorophenol red-β-D-galactopyranoside)

From: $150.00

Catalog #: C2010005 (1 g)
Chlorophenol red-β-D-galactopyranoside (CPRG) is an excellent beta-galactosidase substrate that surpasses o-nitrophenyl-beta-D-galactopyranoside (ONPG). CPRG is very soluble in water and its chromogenic product (chlorophenol red) has an extinction coefficient that is 21-fold higher than that of ONPG (o-nitrophenol). As a result, CPRG can be used in highly sensitive beta-galactosidase-based assays and detection methods. Absorbance of chlorophenol red can be measured at 570-595 nm.

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Description

CPRG (Chlorophenol red-β-D-galactopyranoside)

Product Data Sheet Download PDF
MSDS Download PDF
Catalog number: C2010005
Lot number: 0119C0005
Expiration date: 5/9/23
Molecular Weight: 607.4 g/mol (sodium salt)
Purity: > 95% by HPLC
Package Size: 1 g and 5 g
Appearance: Orange Yellow Powder
Storage: -20°. Avoid repeated freeze-thaw.

 

References

  • Els Vanderperren Wim Demaré Ronny Blust Kris Cooreman Peter Bossier. Oestrogenic activity of CPRG (chlorophenol red-β-D-galactopyranoside), a β-galactosidase substrate commonly used in recombinant yeast oestrogenic assays. Biomarkers. 2001;6(5):375-80.
  • Liaw J, Chang SF, Hsiao FC. In vivo gene delivery into ocular tissues by eye drops of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) polymeric micelles. Gene Ther. 2001 Jul;8(13):999-1004.
  • De Boever P, Demaré W, Vanderperren E, Cooreman K, Bossier P, Verstraete W. Optimization of a yeast estrogen screen and its applicability to study the release of estrogenic isoflavones from a soygerm powder. Environ Health Perspect. 2001 Jul;109(7):691-7.
  • Warolen M, Walum E, Wilcke M. Detection of insulin-regulated GLUT4-translocation by the insertion of a protein C epitope in L6 myoblasts.J Biochem Biophys Methods. 2002 Apr 18;51(2):121-7.
  • Buller CJ, Zang XP, Howard EW, Pento JT. Measurement of beta-galactosidase tissue levels in a tumor cell xenograft model. Methods Find Exp Clin Pharmacol. 2003 Nov;25(9):713-6.
  • Esperandim VR, da Silva Ferreira D, Toldo MP, Saraiva J, Augusto MB, de Albuquerque S. New method for quantification of Trypanosoma cruzi in animal’s tissue in the chronic phase of experimental Chagas’ disease. Parasitol Res. 2010 May;106(6):1471-3.
  • Shin HJ. Agarose-gel-immobilized recombinant bacterial biosensors for simple and disposable on-site detection of phenolic compounds. Appl Microbiol Biotechnol. 2012 Mar;93(5):1895-904.
  • Cho JH, Lee DY, Lim WK, Shin HJ. A recombinant Escherichia coli biosensor for detecting polycyclic aromatic hydrocarbons in gas and aqueous phases. Prep Biochem Biotechnol. 2014;44(8):849-60.
  • Burnham S, Hu J, Anany H, Brovko L, Deiss F, Derda R, Griffiths MW. Towards rapid on-site phage-mediated detection of generic Escherichia coli in water using luminescent and visual readout. Anal Bioanal Chem. 2014 Sep;406(23):5685-93

 

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