L-Leucine p-nitroanilide

Description

L-Leucine p-nitroanilide
Catalog number: MDP0023
CAS Number: 94367-21-2
Sequence: L-Leucine p-nitroanilide
Amount: 100 mg
Molecular Weight: 251.2 g/mol
Supplied as: Powder
Applications: molecular tool for various biochemical applications
Storage: -20 °C
WARNING: For in vitro Research Use Only. NOT for use on humans or animals.
Grade: Biotechnology grade. All products are highly pure.

References:
1: Filippova IYu, Lysogorskaya EN, Oksenoit ES, Rudenskaya GN, Stepanov VM. L-Pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide–a chromogenic substrate for thiol proteinase assay Anal Biochem. 1984 Dec;143(2):293-7.
2: Lin SH, Van Wart HE. Effect of cryosolvents and subzero temperatures on the hydrolysis of L-leucine-p-nitroanilide by porcine kidney leucine aminopeptidase Biochemistry. 1982 Oct 26;21(22):5528-33.
3: Jewgiński M, Haremza K, de Los Santos JM, Es Sbai Z, Oszywa B, Pawełczak M, Palacios F, Latajka R. Phosphinotripeptidic Inhibitors of Leucylaminopeptidases Int J Mol Sci. 2021 May 11;22(10):5090.
4: Murata K, Iba Y, Inoue Y, Kimura A. L-leucine and its analogue: specific inhibitors for S-benzyl-L-cysteine-p-nitroanilide-hydrolyzing enzyme in Escherichia coli B Biochem Biophys Res Commun. 1988 Jun 16;153(2):767-72.
5: Lapp CA, O’Conner JL. Peptidase activity in the hypothalamus of the rat: utilization of leucine-p-nitroanilide to monitor the activity degrading luteinizing hormone releasing hormone Biol Reprod. 1984 May;30(4):848-54.
6: Shimasue A, Sugimoto Y, Tsubokura T. [No blank method using L-leucine-p-nitroanilide as substrate for the assay of serum leucine aminopeptidase (author’s transl)] Rinsho Byori. 1977 Feb;25(2):157-60.
7: Hegazi FZ, Abo-Elnaga IG. Proteolytic activity of crude cell-free extract of Lactobacillus casei and Lactobacillus plantarum Nahrung. 1987;31(3):225-32.
8: Bozić N, Vujcić Z. Detection and quantification of leucyl aminopeptidase after native electrophoresis using leucine-p-nitroanilide Electrophoresis. 2005 Jun;26(12):2476-80.
9: Ferreira GM, Kronenberger T, de Almeida ÉC, Sampaio J, Terra CF, Pinto E, Trossini GHG. Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins Molecules. 2019 Nov 29;24(23):4369.
10: Bayliss ME, Prescott JM. Modified activity of Aeromonas aminopeptidase: metal ion substitutions and role of substrates Biochemistry. 1986 Dec 2;25(24):8113-7.

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